Transferrin Family Genes in the Brown Planthopper, Nilaparvata lugens (Hemiptera: Delphacidae) in Response to Three Insecticides

Transferrin Family Genes in the Brown Planthopper, Nilaparvata lugens (Hemiptera: Delphacidae) in Response to Three Insecticides
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褐飞虱、褐飞虱(半翅目:飞虱科)中转铁蛋白家族基因对三种杀虫剂的反应

DOI:
10.1093/jee/tox321
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发表时间:
2017-12
影响因子:
2.2
通讯作者:
Gao Cong-Fen
Gao Cong-Fen
中科院分区:
农林科学2区
文献类型:
--
作者:
Wu Shun-Fan;Li Jian;Zhang Yong;Gao Cong-Fen

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摘要转铁蛋白参与真核生物包括昆虫的铁代谢、免疫、外源性物质耐受和发育。然而,转铁蛋白与杀虫剂毒理学和抗性之间的关系知之甚少。本研究对亚洲水稻主要害虫褐飞虱Nilaparvata lugens(Stål)的3个转铁蛋白家族基因NlTsf1、NlTsf2和NlTsf3进行了鉴定和分析。定量聚合酶链反应结果表明,NlTsf1基因在不同组织中的表达量显著高于其他两个基因。所有这些基因在腹部和头部的表达水平均高于触角、腿、口针和胸部。与对照相比,3个N.在噻嗪酮、吡蚜酮和吡虫啉处理后24 h,褐飞虱转铁蛋白家族基因显著降低。
Abstract Transferrins are involved in iron metabolism, immunity, xenobiotics tolerance, and development in eukaryotic organisms including insects. However, little is known about the relationship between transferrins and insecticide toxicology and resistance. Three transferrin family genes, NlTsf1, NlTsf2, and NlTsf3, of the brown planthopper, Nilaparvata lugens (Stål) (Hemiptera: Delphacidae)a major insect pest of rice field in Asia, had been identified and characterized in this study. Quantitative polymerase chain reaction results demonstrated that NlTsf1 was significantly higher than the other two genes in different tissues. All of them were expressed at higher levels in abdomen and head than in antenna, leg, stylet, and thorax. Compared with the control, the expression of three N. lugens transferrin family genes decreased dramatically 24 h after treatment with buprofezin, pymetrozine and imidacloprid.
DOI: 10.1046/j.1432-1327.1999.00173.x
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