A transferrin gene associated with development and 2-tridecanone tolerance in Helicoverpa armigera.
A transferrin gene associated with development and 2-tridecanone tolerance in Helicoverpa armigera.
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与棉铃虫发育和 2-十三酮耐受性相关的转铁蛋白基因
DOI：
10.1111/imb.12129
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发表时间：
2015-04
影响因子：
2.6
通讯作者：
Gao X
中科院分区：
文献类型：
--
作者：
Zhang L;Shang Q;Lu Y;Zhao Q;Gao X
从棉铃虫（Helicoverpaarmigera）中克隆了一个铁结合转铁蛋白的全长cDNA（2320 bp），命名为HaTrf。推定的HaTrf序列包括670个氨基酸，分子量约为76 kDa。定量PCR结果表明，HaTrf基因在六龄和蛹期的转录水平显著高于其他发育阶段。HaTrf转录本在脂肪体和表皮中比在马氏管中更丰富。与对照组相比，2-十三烷酮处理24 h后HaTrf的表达显著增加。与饲喂绿色荧光蛋白dsRNA的幼虫相比，饲喂HaTrf双链RNA（dsRNA）的幼虫生长明显受到抑制，体重显著下降。HaTrf的RNA干扰也显著增加了幼虫对2-十三烷酮的敏感性。这些结果表明HaTrf可能参与植物次生化学物质的耐受性。
The full-length cDNA (2320 bp) encoding a putative iron-binding transferrin protein from Helicoverpa armigera was cloned and named HaTrf. The putative HaTrf sequence included 670 amino acids with a molecular mass of approximately 76 kDa. Quantitative PCR results demonstrated that the transcriptional level of HaTrf was significantly higher in the sixth instar and pupa stages as compared with other developmental stages. HaTrf transcripts were more abundant in fat bodies and in the epidermis than in malpighian tubules. Compared with the control, the expression of HaTrf increased dramatically 24 h after treatment with 2-tridecanone. Apparent growth inhibition with a dramatic body weight decrease was observed in larvae fed with HaTrf double-stranded RNA (dsRNA), as compared with those fed with green fluorescent protein dsRNA. RNA interference of HaTrf also significantly increased the susceptibility of larvae to 2-tridecanone. These results indicate the possible involvement of HaTrf in tolerance to plant secondary chemicals.
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影响因子：
2.7
作者：
Kell DB
通讯作者：
Kell DB
DOI：
10.1016/j.cbpb.2008.02.009
发表时间：
2008-06-01
影响因子：
2.2
作者：
Kim, Bo Yeon;Lee, Kwang Sik;Jin, Byung Rae
通讯作者：
Jin, Byung Rae
影响因子：
3.8
作者：
do Nascimento, AM;Cuvillier-Hot, V;Hartfelder, K
通讯作者：
Hartfelder, K
影响因子：
2.6
作者：
Kim, J.;Kim, Y.
通讯作者：
Kim, Y.
DOI：
10.1080/10425170701461854
发表时间：
2008-04-01
期刊：
DNA SEQUENCE
影响因子：
--
作者：
Kim, Bo Yeon;Lee, Kwang Sik;Jin, Byung Rae
通讯作者：
Jin, Byung Rae