Insight into the Interaction of Graphene Oxide with Serum Proteins and the Impact of the Degree of Reduction and Concentration.
Insight into the Interaction of Graphene Oxide with Serum Proteins and the Impact of the Degree of Reduction and Concentration.
复制标题
深入了解氧化石墨烯与血清蛋白的相互作用以及还原程度和浓度的影响。
DOI：
10.1021/acsami.5b01874
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发表时间：
2015-06
期刊：
影响因子：
--
通讯作者：
Qiang Peng
中科院分区：
文献类型：
--
作者：
Wei XQ;Hao LY;Shao XR;Zhang Q;Jia XQ;Zhirong Zhang;Lin Yunfeng;Qiang Peng
氧化石墨烯（GO）及其还原形态（rGO）作为一种新型纳米材料，因其优异的性能而受到全球的广泛关注。然而，缺乏对它们与生物大分子相互作用的全面了解，极大地限制了它们在生物医学上的应用。本研究旨在系统研究氧化石墨烯/还原氧化石墨烯与血清蛋白相互作用时的性质变化，以及它们的还原程度和暴露浓度如何影响这种相互作用，并分析这种相互作用可能产生的生物医学影响。我们发现蛋白质在氧化石墨烯/还原氧化石墨烯上的吸附是自发而迅速的，导致大小、zeta电位和形态的显著变化。与氧化石墨烯相比，氧化石墨烯表现出更高的猝灭血清蛋白固有荧光的能力，并呈浓度依赖性。蛋白质的吸附效率和相关蛋白质的类型不同，取决于石墨烯的还原程度和浓度。我们的研究结果表明，在使用氧化石墨烯/还原氧化石墨烯进行药物递送之前，评估潜在的蛋白质吸附非常重要，因为蛋白质吸附后物理化学性质的变化将对这些递送系统的安全性和有效性产生重大影响。另一方面，这种相互作用也可用于某些蛋白质的分离、纯化或递送。
As novel applied nanomaterials, both graphene oxide (GO) and its reduced form (rGO) have attracted global attention, because of their excellent properties. However, the lack of comprehensive understanding of their interactions with biomacromolecules highly limits their biomedical applications. This work aims to initiate a systematic study on the property changes of GO/rGO upon interaction with serum proteins and on how their degree of reduction and exposure concentration affect this interaction, as well as to analyze the possible biomedical impacts of the interaction. We found that the adsorption of proteins on GO/rGO occurred spontaneously and rapidly, leading to significant changes in size, zeta potential, and morphology. Compared to rGO, GO showed a higher ability in quenching intrinsic fluorescence of serum proteins in a concentration-dependent manner. The protein adsorption efficiency and the types of associated proteins varied, depending on the degree of reduction and concentration of graphene. Our findings indicate the importance of evaluating the potential protein adsorption before making use of GO/rGO in drug delivery, because the changed physicochemical properties after protein adsorption will have significant impacts on safety and effectiveness of these delivery systems. On the other hand, this interaction can also be used for the separation, purification, or delivery of certain proteins.
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影响因子：
78.8
作者：
Scheinberg, David A.;Villa, Carlos H.;Escorcia, Freddy E.;McDevitt, Michael R.
通讯作者：
McDevitt, Michael R.
影响因子：
17.1
作者：
Lundqvist, Martin;Stigler, Johannes;Dawson, Kenneth
通讯作者：
Dawson, Kenneth
影响因子：
15
作者：
HUMMERS, WS;OFFEMAN, RE
通讯作者：
OFFEMAN, RE
影响因子：
13.3
作者：
Yan Zhang;Jingyan Zhang;Xuelei Huang;Xuejiao Zhou;Haixia Wu;Shouwu Guo
通讯作者：
Yan Zhang;Jingyan Zhang;Xuelei Huang;Xuejiao Zhou;Haixia Wu;Shouwu Guo
影响因子：
4.9
作者：
Kurapati, Rajendra;Raichur, Ashok M.
通讯作者：
Raichur, Ashok M.