Tumor necrosis factor alpha maintains the viability of murine epidermal Langerhans cells in culture, but in contrast to granulocyte/macrophage colony-stimulating factor, without inducing their functional maturation.
Tumor necrosis factor alpha maintains the viability of murine epidermal Langerhans cells in culture, but in contrast to granulocyte/macrophage colony-stimulating factor, without inducing their functional maturation.
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肿瘤坏死因子α维持鼠表皮兰格汉细胞在培养中的生存能力，但与粒细胞/巨噬细胞刺激因子相反，而没有诱导其功能成熟。
DOI：
10.1084/jem.171.1.159
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发表时间：
1990-01-01
影响因子：
15.3
通讯作者：
SCHULER, G
中科院分区：
文献类型：
--
作者：
KOCH, F;HEUFLER, C;KAMPGEN, E;SCHNEEWEISS, D;BOCK, G;SCHULER, G
新鲜分离的鼠表皮Langerhans细胞（LC）是静息T细胞的弱刺激剂，但在培养2-3 d培养物以及其他表皮细胞时，它们的刺激能力为10-30倍。 LC的这种成熟是由两种角质细胞产物介导的。粒细胞/巨噬细胞刺激因子（GM-CSF）保持生存能力并增加功能。仅IL-1并不能使LC保持活力，但是当与GM-CSF结合使用时，可以进一步增强其刺激活性。现在，我们已经搜索了一种将LC保持在可行但功能不成熟的状态的细胞因子。当LC（富含75％的LC）在GM-CSF（2 ng/ml）或鼠（TNF-Alpha）（62 U/ml的高原效应）的存在下培养时，72 h是相同的。然而，在鼠TNF-α中培养的LC在刺激静息T细胞中的活性降低了10-30倍。一系列实验表明，这种现象并不是由于GM-CSF的诱导不足，诱导抑制因子或TNF-Alpha的毒性作用。有趣的是，观察到的TNF-α活性表现出物种的偏好，因为人类TNF-α在可比剂量下没有活性。我们已经观察到TNF-Alpha对LC体外的意外作用。尽管我们发现新鲜制备的表皮细胞表达TNF-Alpha mRNA，但仍需要进一步的研究来确定TNF-Alpha是否通过将居民LC保持在可行但功能不成熟的状态中，是否在体内起作用。
Freshly isolated murine epidermal Langerhans cells (LC) are weak stimulators of resting T cells but increase their stimulatory capacity 10-30-fold upon 2-3 d of culture together with other epidermal cells. This maturation of LC is mediated by two keratinocyte products. Granulocyte/macrophage colony-stimulating factor (GM-CSF) maintains viability and increases function. IL-1 alone does not keep LC alive, but when combined with GM-CSF further enhances their stimulatory activity. We have now searched for a cytokine that would keep LC in a viable, but functionally immature state. When LC (enriched to greater than 75%) were cultured in the presence of GM-CSF (2 ng/ml) or murine (TNF-alpha) (plateau effect at 62 U/ml), the recovery of viable LC after 72 h was identical. The LC cultured in murine TNF-alpha, however, were 10-30 times less active in stimulating resting T cells. A series of experiments demonstrated that this phenomenon was not due to the induction of insufficient amounts of GM-CSF, the induction of a suppressor factor, or a toxic effect of TNF-alpha. Interestingly, the observed TNF-alpha activity exhibited a species preference, as human TNF-alpha was not active at comparable doses. We have observed an unexpected effect of TNF-alpha on LC in vitro. Though we found that freshly prepared epidermal cells express TNF-alpha mRNA, further studies are needed to establish whether TNF-alpha plays a role in vivo by keeping resident LC in a viable, but functionally immature state.
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影响因子：
64.8
作者：
LOMEDICO, PT;GUBLER, U;MIZEL, SB
通讯作者：
MIZEL, SB
DOI：
10.1084/jem.161.3.526
发表时间：
1985-03-01
期刊：
The Journal of experimental medicine
影响因子：
--
作者：
Schuler G;Steinman RM
通讯作者：
Steinman RM
DOI：
10.1084/jem.169.3.1153
发表时间：
1989-03-01
期刊：
The Journal of experimental medicine
影响因子：
--
作者：
Romani N;Inaba K;Puré E;Crowley M;Witmer-Pack M;Steinman RM
通讯作者：
Steinman RM
影响因子：
64.5
作者：
CLEVELAND, DW;LOPATA, MA;KIRSCHNER, MW
通讯作者：
KIRSCHNER, MW
影响因子：
2.9
作者：
CHIRGWIN, JM;PRZYBYLA, AE;RUTTER, WJ
通讯作者：
RUTTER, WJ