Comparative study on intestinal bacterial communities of Boleophthalmus pectinirostris and Periophthalmus magnuspinnatus with different sexes and feeding strategies
Comparative study on intestinal bacterial communities of Boleophthalmus pectinirostris and Periophthalmus magnuspinnatus with different sexes and feeding strategies
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不同性别大弹涂鱼和大刺围眼鱼肠道细菌群落及饲养策略的比较研究
DOI：
10.1007/s13213-018-1324-4
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发表时间：
2018-01
影响因子：
3
通讯作者：
Zhuang P
中科院分区：
文献类型：
--
作者：
Ma RR;Wang SK;Zhao F;Xu C;Ji Y;Song C;Wang Y;Zhuang P
潮间带泥滩是独特的高产生态系统。长江口潮间带泥滩上常见的鱼类有大眼鲆和大眼鲆。它们具有重要的生态功能和不同的摄食策略。本研究研究了不同性别、不同摄食策略的野生pectinrostris和P. magnuspinnatus在繁殖季节的肠道微生物多样性和结构。收集pectinrostris和P. magnuspinnatus个体（雌雄比例为1:1）的肠道样本，进行高通量DNA测序。结果表明，变形菌门在4个类群中均为最优势门，其中雄虫占73.5%，雌虫占52.6%；马格斯皮纳图虫占40.2%，雌虫占40.9%。变形菌门的气单胞菌、希瓦氏菌、盐单胞菌和不动杆菌是所有样品组的优势属，占10个优势属的62.13%。杂食性magnuspinnatus肠道菌群多样性显著高于草食性B. pectinrostris （P < 0.05）。包括未加权UniFrac距离的PCoA和UPGMA在内的Beta多样性表明，B. pectinrostris样品和P. magnuspinnatus样品聚集在一起，表明取食习惯对微生物群落结构的影响比性别的影响更大。
Intertidal mudflats are unique, highly productive ecosystems. Boleophthalmus pectinirostris and Periophthalmus magnuspinnatus are common fish species that are distributed in the intertidal mudflats of the Yangtze Estuary in China. They perform important ecological functions and have different feeding strategies. Herein, we studied the intestinal microbial diversity and structure of wild B. pectinirostris and P. magnuspinnatus with different sexes and feeding strategies during their breeding season. Gut samples of B. pectinirostris and P. magnuspinnatus individuals (female:male ratio = 1:1) were collected and subjected to high-throughput DNA sequencing. The results showed Proteobacteria was the most dominant phylum in all the four sample groups: 73.5% in the males and 52.6% in the females of B. pectinirostris and 40.2% in the males and 40.9% in the females of P. magnuspinnatus. Aeromonas, Shewanella, Halomonas, and Acinetobacter of the phylum Proteobacteria were dominant genera in all the sample groups and accounted for 62.13% of the ten dominant genera. The diversity of the intestinal microflora in the omnivorous P. magnuspinnatus was significantly higher (P < 0.05) than that in the herbivorous B. pectinirostris. Beta diversity, including PCoA and UPGMA of unweighted UniFrac distances, showed that B. pectinirostris samples were clustered together, and P. magnuspinnatus samples were clustered together, implying the effect of the feeding habits on the microbial community structure is more considerable than that of sex.
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影响因子：
2.6
作者：
Mrazek, J.;Strosova, L.;Kopecny, J.
通讯作者：
Kopecny, J.
影响因子：
16.6
作者：
Sanders JG;Beichman AC;Roman J;Scott JJ;Emerson D;McCarthy JJ;Girguis PR
通讯作者：
Girguis PR
DOI：
10.1126/science.1198719
发表时间：
2011-05-20
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Muegge BD;Kuczynski J;Knights D;Clemente JC;González A;Fontana L;Henrissat B;Knight R;Gordon JI
通讯作者：
Gordon JI
影响因子：
11
作者：
Stephens, W. Zac;Burns, Adam R.;Bohannan, Brendan J. M.
通讯作者：
Bohannan, Brendan J. M.
影响因子：
3.6
作者：
Romero, Jaime;Navarrete, Paola
通讯作者：
Navarrete, Paola