A higher frequency of circulating IL-22(+)CD4(+) T cells in Chinese patients with newly diagnosed Hashimoto's thyroiditis.
A higher frequency of circulating IL-22(+)CD4(+) T cells in Chinese patients with newly diagnosed Hashimoto's thyroiditis.
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在中国新诊断的桥本甲状腺炎患者中，循环IL-22（+）CD4（+）T细胞的循环频率更高。
DOI：
10.1371/journal.pone.0084545
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发表时间：
2014
期刊：
影响因子：
3.7
通讯作者：
Jiang YF
中科院分区：
文献类型：
--
作者：
Guo H;Peng D;Yang XG;Wang Y;Xu BC;Ni JS;Meng W;Jiang YF
IL-22和IL-17 A与自身免疫性疾病的发病机制有关。然而，IL-22+和IL-17A + CD4 + T细胞在桥本甲状腺炎（HT）发病机制中的作用尚未完全了解。本研究调查了HT患者血清IL-22和IL-17A水平，并确定了HT患者循环IL-22 + CD4 + T细胞的频率，以了解它们在HT发病机制中的作用。采用酶联免疫吸附试验（ELISA）和流式细胞术检测17例HT患者和17例健康对照者（HC）血清IL-22、IL-17A和IFN-γ水平及外周血IL-22 + CD4+和IL-17A + CD4 + T细胞频率。采用荧光酶免疫法和放射免疫法测定血清游离三碘甲状腺原氨酸（FT4）、游离甲状腺素（FT3）、促甲状腺激素（TSH）、抗甲状腺过氧化物酶（TPO）和抗甲状腺球蛋白抗体（TgAb）水平。HT组外周血IL-22 + CD4+和IL-17 + CD4 + T细胞百分率（p <0.0001，p <0.0001）及血清IL-22、IL-17 A和IFN-γ水平（p <0.0001，p <0.0001，p = 0.0210）均显著高于HC组。 HT患者IL-22 + CD4 + T细胞百分比与Th17细胞（r = 0.8815，p <0.0001）和IL-17A + IL-22 + CD4 + T细胞（r = 0.8914，p <0.0001）呈正相关，但与Th1细胞呈负相关（r =-0.6110，p <0.0092）。 在HT患者中，Th22细胞、Th17细胞和IL-17A + IL-22 + CD4 + T细胞的百分比与血清TSH水平呈负相关（分别为r =-0.8402，p <0.0001; r =-0.8589，p <0.0001; r =-0.8289，p <0.0001）。 高频率的IL-22 + CD4+和IL-17A + CD4 + T细胞可能与中国HT患者的发展有关。
IL-22 and IL-17A are implicated in the pathogenesis of autoimmune diseases. However, the role of IL-22+ and IL-17A+ CD4+ T cells in the pathogenesis of Hashimoto’s thyroiditis (HT) is not fully understood. This study investigates serum IL-22 and IL-17A levels and determines the frequency of circulating IL-22+ CD4+ T cells in HT patients to understand their roles in the pathogenesis of HT. The levels of serum IL-22, IL-17A and IFN-γ and the frequency of circulating IL-22+CD4+ and IL-17A+CD4+ T cells in 17 HT patients and 17 healthy controls (HC) were determined by enzyme-linked immunosorbent assay (ELISA) and flow cytometry. The levels of serum free triiodothyronine (FT4), free thyroxine (FT3), thyroid stimulating hormone (TSH), anti-thyroid peroxidase (TPO) and anti-thyroglobulin antibodies (TgAb) by chemiluminescent enzyme immunoassay and radioimmunoassay. The percentages of circulating IL-22+CD4+ and IL-17+CD4+ T cells (p<0.0001, p<0.0001) and the levels of serum IL-22, IL-17A and IFN-γ (p<0.0001, p<0.0001, p = 0.0210) in the HT patients were significantly higher than that in the HC. The percentages of IL-22+CD4+ T cells were positively correlated with Th17 cells (r = 0.8815, p<0.0001) and IL-17A+IL-22+CD4+ T cells (r = 0.8914, p<0.0001), but were negatively correlated with Th1 cells (r = −0.6110, p<0.0092) in the HT patients. The percentages of Th22 cells, Th17 cells and IL-17A+IL-22+CD4+ T cells were negatively correlated with the levels of serum TSH in the HT patients (r = −0.8402, p<0.0001; r = −0.8589, p<0.0001; r = −0.8289 p<0.0001, respectively). A higher frequency of circulating IL-22+CD4+ and IL-17A+CD4+ T cells may be associated with the development of HT in Chinese patients.
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影响因子：
15.8
作者：
Baccarelli A;Giacomini SM;Corbetta C;Landi MT;Bonzini M;Consonni D;Grillo P;Patterson DG;Pesatori AC;Bertazzi PA
通讯作者：
Bertazzi PA
影响因子：
5.8
作者：
Figueroa-Vega, Nicte;Alfonso-Perez, Manuel;Marazuela, Monica
通讯作者：
Marazuela, Monica
影响因子：
30.5
作者：
Duhen, Thomas;Geiger, Rebekka;Sallusto, Federica
通讯作者：
Sallusto, Federica
DOI：
10.1590/s0100-879x2012007500168
发表时间：
2012-12
期刊：
Brazilian journal of medical and biological research = Revista brasileira de pesquisas medicas e biologicas
影响因子：
--
作者：
Qin Q;Liu P;Liu L;Wang R;Yan N;Yang J;Wang X;Pandey M;Zhang JA
通讯作者：
Zhang JA
影响因子：
7.7
作者：
Kallmann, BA;Huther, M;Kolb, H
通讯作者：
Kolb, H