An invariant T cell receptor alpha chain is used by a unique subset of major histocompatibility complex class I-specific CD4+ and CD4-8- T cells in mice and humans.
An invariant T cell receptor alpha chain is used by a unique subset of major histocompatibility complex class I-specific CD4+ and CD4-8- T cells in mice and humans.
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不变的T细胞受体α链由小鼠和人类的主要组织相容性复合物I类CD4+和CD4-8-T细胞的独特子集使用。
DOI：
10.1084/jem.180.3.1097
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发表时间：
1994-09-01
影响因子：
15.3
通讯作者：
Bendelac, Albert
中科院分区：
文献类型：
--
作者：
Lantz, Olivier;Bendelac, Albert
小鼠胸腺包含一个成熟的T细胞亚群，通过几个特征与主流胸腺细胞区分开来。它仅限于T细胞受体(TCR)Vβ基因对Vβ8、Vβ7和Vβ2的使用，其表面表型为激活/记忆细胞。它带有自然杀手NK1.1表面标记。此外，尽管它完全由CD4+和CD4-8-细胞组成，但它在胸腺中的选择完全取决于造血细胞表达的主要组织相容性复合体(MHC)I类。CD8的强制持久性实际上是一种负面选择。在这里，我们研究了这个子集的TCR谱系，发现虽然β链V-D-J连接非常可变，但大多数TCR使用单个不变的阿尔法链Vα14-J281。Vα14-J281α链的这种令人惊讶的限制使用依赖于MHC I类表达，但与MHC单倍型无关。在人类中，也可以发现包括CD4-8细胞在内的类似的不寻常群体，使用惊人的同源、不变的阿尔法链Vα24-JQ。因此，这种独特的Vα-Jα组合在两个物种中都是保守的，赋予了某些共享的非多态MHC I类/多肽自配体(S)的特异性。这意味着它所定义的T细胞亚群具有特殊而重要的作用，可能与其在体外和体内初次刺激时分泌包括白介素4在内的大量淋巴因子的独特能力有关。
The mouse thymus contains a mature T cell subset that is distinguishable from the mainstream thymocytes by several characteristics. It is restricted in its usage of T cell receptor (TCR) V beta genes to V beta 8, V beta 7, and V beta 2. Its surface phenotype is that of activated/memory cells. It carries the natural killer NK1.1 surface marker. Furthermore, though it consists entirely of CD4+ and CD4-8- cells, its selection in the thymus depends solely upon major histocompatibility complex (MHC) class I expression by cells of hematopoietic origin. Forced persistence of CD8, in fact, imparts negative selection. Here, we have studied the TCR repertoire of this subset and found that, whereas the beta chain V-D-J junctions are quite variable, a single invariant alpha chain V alpha 14-J281 is used by a majority of the TCRs. This surprisingly restricted usage of the V alpha 14-J281 alpha chain is dependent on MHC class I expression, but independent of the MHC haplotype. In humans, a similar unusual population including CD4-8- cells can also be found that uses a strikingly homologous, invariant alpha chain V alpha 24-JQ. Thus, this unique V alpha-J alpha combination has been conserved in both species, conferring specificity to some shared nonpolymorphic MHC class I/peptide self-ligand(s). This implies that the T cell subset that it defines has a specialized and important role, perhaps related to its unique ability to secrete a large set of lymphokines including interleukin 4, upon primary stimulation in vitro and in vivo.
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影响因子：
56.9
作者：
GRUSBY, MJ;JOHNSON, RS;GLIMCHER, LH
通讯作者：
GLIMCHER, LH
影响因子：
15.3
作者：
HAYAKAWA, K;LIN, BT;HARDY, RR
通讯作者：
HARDY, RR
影响因子：
15.3
作者：
BOITEL, B;ERMONVAL, M;ACUTO, O
通讯作者：
ACUTO, O
影响因子：
64.8
作者：
ASARNOW, DM;CADO, D;RAULET, DH
通讯作者：
RAULET, DH
DOI：
10.1073/pnas.87.14.5248
发表时间：
1990-07-01
影响因子：
11.1
作者：
KOSEKI, H;IMAI, K;TANIGUCHI, M
通讯作者：
TANIGUCHI, M