Genome-Wide Analysis of Glycine soja Response Regulator GsRR Genes Under Alkali and Salt Stresses.
Genome-Wide Analysis of Glycine soja Response Regulator GsRR Genes Under Alkali and Salt Stresses.
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碱盐胁迫下大豆反应调节因子 GsRR 基因的全基因组分析
DOI：
10.3389/fpls.2018.01306
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发表时间：
2018
影响因子：
5.6
通讯作者：
Zhu Y
中科院分区：
文献类型：
--
作者：
Chen C;Liu A;Ren H;Yu Y;Duanmu H;Duan X;Sun X;Liu B;Zhu Y
土壤盐碱化是植物生长面临的一个严峻挑战因素。野生大豆（Glycine soja）具有对盐碱胁迫良好耐受的优良特性，近期研究发现响应调节因子家族基因参与多种非生物胁迫。对野生大豆中所有响应调节因子基因进行基因组和转录组分析，将有助于深入了解它们在植物胁迫响应中的功能。在本研究中，我们鉴定并描述了总共56个野生大豆响应调节因子（GsRR）基因。系统发育分析表明，GsRR基因可分为5个亚类（A1、A2、B1、B2和C）。我们进一步研究了GsRRs的染色体位置、基因重复和保守结构域。此外，对GsRR转录图谱的聚类分析揭示了在碱胁迫下5种不同的表达模式。A1和A2亚类的转录水平显著高于B亚类。此外，实时定量PCR结果证实GsRR基因也受到盐胁迫的显著影响。值得注意的是，A1亚类中的GsRR2a在盐胁迫下与碱胁迫下呈现相反的表达模式。而且，在拟南芥中过表达GsRR2a显著提高了对碱胁迫的耐受性，但对盐胁迫没有作用。这些结果表明GsRR基因在应对盐和碱胁迫中具有重要作用，也为进一步研究GsRR家族基因的功能特性提供了有价值的线索。
Soil salt-alkalization is a dramatic challenging factor for plant growth. Wild soybean (Glycine soja) exhibits a favorable trait of superior tolerance to salt-alkali stress, and recent discoveries show that response regulator family genes are involved in diverse abiotic stresses. Genomic and transcriptomic analyses of all response regulator genes in wild soybean will provide insight into their function in plant stress response. In this study, we identified and characterized a total of 56 Glycine soja response regulator (GsRR) genes. Phylogenetic analysis suggested that GsRR genes could be classified into five subclasses (A1, A2, B1, B2, and C). We further investigated the chromosome locations, gene duplications and conserved domains of the GsRRs. Furthermore, the clustering analysis of GsRR transcript profiles revealed five different expression patterns under alkali stress. The A1 and A2 subclasses display significantly higher transcriptional levels than the B subclass. In addition, quantitative real-time PCR results verified that the GsRR genes were also significantly influenced by salt stress. Notably, GsRR2a in the A1 subclass showed opposite expression patterns under salt stress comparing with alkali stress. Moreover, overexpression of GsRR2a in Arabidopsis significantly improved the tolerance to alkali stress, but not salt stress. These results suggest the important roles of GsRR genes in response to salt and alkaline stresses, and also provide valuable clues for further functional characterization of GsRR family genes.
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GsCHX19.3是来自野生大豆的阳离子/H交换超家族的成员，有助于高盐度和碳酸盐碱性耐受性
影响因子：
4.6
作者：
Jia B;Sun M;DuanMu H;Ding X;Liu B;Zhu Y;Sun X
通讯作者：
Sun X
影响因子：
2.9
作者：
Lam-Son Phan Tran;Shinozaki, Kazuo;Yamaguchi-Shinozaki, Kazuko
通讯作者：
Yamaguchi-Shinozaki, Kazuko
模因套件：用于发现和搜索的工具。
影响因子：
14.9
作者：
Bailey TL;Boden M;Buske FA;Frith M;Grant CE;Clementi L;Ren J;Li WW;Noble WS
通讯作者：
Noble WS
影响因子：
7.2
作者：
Clough, SJ;Bent, AF
通讯作者：
Bent, AF
影响因子：
5.8
作者：
Larkin, M. A.;Blackshields, G.;Higgins, D. G.
通讯作者：
Higgins, D. G.