Spm1, a stress‐activated MAP kinase that regulates morphogenesis in S.pombe
Spm1, a stress‐activated MAP kinase that regulates morphogenesis in S.pombe
复制标题
Spm1，一种应激激活的 MAP 激酶，可调节粟酒裂殖酵母的形态发生
作者：
T. Zaitsevskaya;Jonathan A. Cooper
从裂殖酵母裂殖酵母中分离出编码新 MAP 激酶家族成员 Spm1 的基因。 Spm1 的过量产生会抑制增殖。 spm1+ 基因的破坏会干扰细胞分离和形态发生。在营养限制、高渗应激或升高温度的条件下，spm1Δ细胞生长为短分支丝，其中细胞壁和隔膜增厚，表明极化生长和细胞壁重塑存在缺陷。在高渗透压下，spm1Δ 细胞无法形成集落。 Spm1 蛋白被酪氨酸磷酸化并响应渗透压和热应激而被激活，这与 Spm1 在适应这些条件中的作用一致。另外两种已知的粟酒裂殖酵母 MAP 激酶是性分化和孢子形成所需的 Spk1，以及在高渗条件下激活的 Spc1/Sty1/Phh1。然而，spm1Δ突变体表型的独特特征和直接生化分析表明Spm1并不依赖于其他已知的MAP激酶途径。我们的结果证明存在一种新的 MAP 激酶途径，该途径可调节细胞壁重塑和胞质分裂以应对环境压力。
A gene encoding a novel MAP kinase family member, Spm1, was isolated from the fission yeast Schizosaccharomyces pombe. Overproduction of Spm1 inhibits proliferation. Disruption of the spm1+ gene interferes with cell separation and morphogenesis. Under conditions of nutrient limitation, hypertonic stress or elevated temperature, spm1Δ cells grow as short branched filaments in which the cell walls and septa are thickened, suggesting defects in polarized growth and cell wall remodeling. At high osmolarity, spm1Δ cells fail to form colonies. The Spm1 protein is tyrosine phosphorylated and activated in response to osmotic and heat stress, consistent with a role for Spm1 in adaptation to these conditions. Two other S.pombe MAP kinases are known, Spk1, required for sexual differentiation and sporulation, and Spc1/Sty1/Phh1, which is activated in hypertonic conditions. However, the distinctive features of the spm1Δ mutant phenotype and direct biochemical assays suggest that Spm1 does not lie on other known MAP kinase pathways. Our results demonstrate the existence of a new MAP kinase pathway that regulates cell wall remodeling and cytokinesis in response to environmental stresses.
影响因子：
10.5
作者：
ROBERTS, RL;FINK, GR
通讯作者：
FINK, GR
影响因子：
56.9
作者：
LIU, HP;STYLES, CA;FINK, GR
通讯作者：
FINK, GR
影响因子：
7.5
作者：
LEVIN, DE;ERREDE, B
通讯作者：
ERREDE, B