Plasmodium falciparum field isolates use complement receptor 1 (CR1) as a receptor for invasion of erythrocytes.
Plasmodium falciparum field isolates use complement receptor 1 (CR1) as a receptor for invasion of erythrocytes.
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DOI：
10.1016/j.molbiopara.2011.01.005
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发表时间：
2011-05
影响因子：
1.5
通讯作者：
Stoute, Jose A.
中科院分区：
文献类型：
--
作者：
Awandare, Gordon A.;Spadafora, Carmenza;Moch, J. Kathleen;Dutta, Sheetij;Haynes, J. David;Stoute, Jose A.
大多数恶性疟原虫菌株通过与糖蛋白上的唾液酸（SA）相互作用侵入红细胞。然而，我们最近报道了补体受体1 （CR1）是许多恶性疟原虫实验室菌株的sa独立入侵受体。为了确定CR1在恶性疟原虫现场分离株红细胞侵袭中的作用，我们测试了从肯尼亚儿童中获得的8株分离株。所有被检测的寄生虫都能够以sa独立的方式入侵，并且抗CR1和可溶性CR1 （sCR1）几乎完全阻断神经氨酸酶处理的红细胞的入侵。此外，抗cr1和sCR1在大多数分离株中部分抑制完整红细胞的侵袭。恶性疟原虫AMA-1高变区测序结果显示，各分离株间存在较大差异。这些数据表明，CR1介导恶性疟原虫分离株sa非依赖性红细胞侵袭。
A majority of Plasmodium falciparum strains invade erythrocytes through interactions with sialic acid (SA) on glycophorins. However, we recently reported that complement receptor 1 (CR1) is a SA-independent invasion receptor of many laboratory strains of Plasmodium falciparum. To determine the role of CR1 in erythrocyte invasion among P. falciparum field isolates, we tested eight isolates obtained from children in Kenya. All the parasites examined were capable of invading in a SA-independent manner, and invasion of neuraminidase-treated erythrocytes was nearly completely blocked by anti-CR1 and soluble CR1 (sCR1). In addition, anti-CR1 and sCR1 partially inhibited invasion of intact erythrocytes in a majority of isolates tested. Sequencing of the hypervariable region of P. falciparum AMA-1 showed considerable diversity among all the isolates. These data demonstrate that CR1 mediates SA-independent erythrocyte invasion in P. falciparum field isolates.
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