Markers of epidemiological success of methicillin-resistant Staphylococcus aureus isolates in European populations.
Markers of epidemiological success of methicillin-resistant Staphylococcus aureus isolates in European populations.
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DOI：
10.1016/j.cmi.2023.05.015
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发表时间：
2023-09
影响因子：
14.2
通讯作者：
Lindsay, Jodi A.
中科院分区：
文献类型：
--
作者：
Baede, Valerie O.;Gupta, Arya;Knight, Gwenan M.;Schouls, Leo M.;Laing, Ken;Tavakol, Mehri;Barray, Anais;de Vlas, Sake J.;de Vos, Anneke S.;Hendrickx, Antoni P. A.;Khan, Madeeha;Kretzschmar, Mirjam E.;van Wamel, Willem J. B.;Lina, Gerard;Vandenesch, Francois;Vos, Margreet C.;Witney, Adam A.;Rasigade, Jean-Philippe;Lindsay, Jodi A.
耐甲氧西林金黄色葡萄球菌（MRSA）感染给卫生系统带来了相当大的负担，但MRSA的全球发病率和流行病学存在显着差异。MACOTRA联盟的目的是使用来自法国、荷兰和英国的代表性MRSA收集物来鉴定欧洲MRSA分离株流行成功的细菌标志物。在联盟会议上定义了成功的操作定义，以组成成功和散发性MRSA分离株的平衡菌株集合。分离株进行抗菌药物敏感性测试和全基因组测序，基因进行了鉴定和构建系统发育树。流行病学成功的标志物，确定了基于基因组的时间尺度单倍型密度分析和线性回归。将ESAC-Net的抗菌药物使用数据与国家MRSA发病率数据进行比较。不同国家MRSA分离株的异质性阻碍了成功的统一操作定义的使用;因此，使用特定国家的方法来建立MACOTRA菌株收集。表型抗生素耐药性在相关MRSA人群中和各国之间存在差异。在时间尺度的单倍型密度分析中，氟喹诺酮类、大环内酯类和莫匹罗星耐药与MRSA成功相关，而庆大霉素、利福平和甲氧苄啶耐药与散发性相关。29个欧洲国家的抗菌药物使用情况差异很大，β-内酰胺类、氟喹诺酮类、大环内酯类和氨基糖苷类的使用与MRSA发病率相关。我们的研究结果是迄今为止将MRSA抗生素耐药谱和抗生素使用与感染发生率和成功克隆传播联系起来的最强有力的结果，这些结果因国家而异。随着时间的推移，统一的分离株收集、分型、耐药性分析和与抗菌药物使用的一致性将有助于进行比较，并进一步支持针对具体国家的干预措施，以减少MRSA负担。
Methicillin-resistant Staphylococcus aureus (MRSA) infections impose a considerable burden on health systems, yet there is remarkable variation in the global incidence and epidemiology of MRSA. The MACOTRA consortium aimed to identify bacterial markers of epidemic success of MRSA isolates in Europe using a representative MRSA collection originating from France, the Netherlands and the United Kingdom. Operational definitions of success were defined in consortium meetings to compose a balanced strain collection of successful and sporadic MRSA isolates. Isolates were subjected to antimicrobial susceptibility testing and whole-genome sequencing; genes were identified and phylogenetic trees constructed. Markers of epidemiological success were identified using genome-based time-scaled haplotypic density analysis and linear regression. Antimicrobial usage data from ESAC-Net was compared with national MRSA incidence data. Heterogeneity of MRSA isolate collections across countries hampered the use of a unified operational definition of success; therefore, country-specific approaches were used to establish the MACOTRA strain collection. Phenotypic antimicrobial resistance varied within related MRSA populations and across countries. In time-scaled haplotypic density analysis, fluoroquinolone, macrolide and mupirocin resistance were associated with MRSA success, whereas gentamicin, rifampicin and trimethoprim resistance were associated with sporadicity. Usage of antimicrobials across 29 European countries varied substantially, and β-lactam, fluoroquinolone, macrolide and aminoglycoside use correlated with MRSA incidence. Our results are the strongest yet to associate MRSA antibiotic resistance profiles and antibiotic usage with the incidence of infection and successful clonal spread, which varied by country. Harmonized isolate collection, typing, resistance profiling and alignment with antimicrobial usage over time will aid comparisons and further support country-specific interventions to reduce MRSA burden.
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影响因子：
3.3
作者：
McCarthy AJ;Loeffler A;Witney AA;Gould KA;Lloyd DH;Lindsay JA
通讯作者：
Lindsay JA
影响因子：
168.9
作者：
DeLeo, Frank R.;Otto, Michael;Kreiswirth, Barry N.;Chambers, Henry F.
通讯作者：
Chambers, Henry F.
DOI：
10.1016/j.ijantimicag.2022.106538
发表时间：
2022-03-08
影响因子：
10.8
作者：
Baede, Valerie O.;David, Michael Z.;Vos, Margreet C.
通讯作者：
Vos, Margreet C.
DOI：
10.1016/s0140-6736(21)02724-0
发表时间：
2022-02-12
期刊：
Lancet (London, England)
影响因子：
--
作者：
Antimicrobial Resistance Collaborators
通讯作者：
Antimicrobial Resistance Collaborators
影响因子：
7
作者：
Holden MT;Hsu LY;Kurt K;Weinert LA;Mather AE;Harris SR;Strommenger B;Layer F;Witte W;de Lencastre H;Skov R;Westh H;Zemlicková H;Coombs G;Kearns AM;Hill RL;Edgeworth J;Gould I;Gant V;Cooke J;Edwards GF;McAdam PR;Templeton KE;McCann A;Zhou Z;Castillo-Ramírez S;Feil EJ;Hudson LO;Enright MC;Balloux F;Aanensen DM;Spratt BG;Fitzgerald JR;Parkhill J;Achtman M;Bentley SD;Nübel U
通讯作者：
Nübel U