Extensive horizontal gene transfer during Staphylococcus aureus co-colonization in vivo.
Extensive horizontal gene transfer during Staphylococcus aureus co-colonization in vivo.
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DOI：
10.1093/gbe/evu214
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发表时间：
2014-09-25
影响因子：
3.3
通讯作者：
Lindsay JA
中科院分区：
文献类型：
--
作者：
McCarthy AJ;Loeffler A;Witney AA;Gould KA;Lloyd DH;Lindsay JA
金黄色葡萄球菌（Staphylococcus aureus）是人类和动物的主要致病菌。S.金黄色葡萄球菌群体的研究表明，不同宿主物种的定殖与携带移动的遗传元件（MGE）有关，特别是能够编码毒力、抗性和免疫逃避途径的噬菌体和质粒。抗微生物S.如果家畜的金黄色葡萄球菌适应有效地定殖于人类，则它们是对人类健康的潜在人畜共患威胁。我们利用实验进化技术和与人类和猪相关的谱系克隆复合体398的变体共同定殖的gnotobiotic仔猪，并使用全基因组测序研究了16天内的生长和遗传变化。人分离株在仔猪上的共定殖比在体外更有效地存活。在共定殖期间，在4小时内检测到MGE从猪转移到人分离株。两种噬菌体和三种质粒的广泛和重复转移导致携带各种各样的移动体的分离株的定殖。后代细菌的全基因组测序显示没有获得核心基因组多态性，突出了MGE的重要性。金黄色葡萄球菌噬菌体重组和整合到新的网站是第一次实验检测。在定殖过程中，无性系不是单一的变异体占优势，而是共存和多样化。出乎意料的是，每只仔猪携带独特的细菌变体群体，表明一旦定殖，仔猪之间的细菌传播有限。我们的数据表明，水平基因转移发生在非常高的频率在体内和显着高于体外检测。
Staphylococcus aureus is a commensal and major pathogen of humans and animals. Comparative genomics of S. aureus populations suggests that colonization of different host species is associated with carriage of mobile genetic elements (MGE), particularly bacteriophages and plasmids capable of encoding virulence, resistance, and immune evasion pathways. Antimicrobial-resistant S. aureus of livestock are a potential zoonotic threat to human health if they adapt to colonize humans efficiently. We utilized the technique of experimental evolution and co-colonized gnotobiotic piglets with both human- and pig-associated variants of the lineage clonal complex 398, and investigated growth and genetic changes over 16 days using whole genome sequencing. The human isolate survived co-colonization on piglets more efficiently than in vitro. During co-colonization, transfer of MGE from the pig to the human isolate was detected within 4 h. Extensive and repeated transfer of two bacteriophages and three plasmids resulted in colonization with isolates carrying a wide variety of mobilomes. Whole genome sequencing of progeny bacteria revealed no acquisition of core genome polymorphisms, highlighting the importance of MGE. Staphylococcus aureus bacteriophage recombination and integration into novel sites was detected experimentally for the first time. During colonization, clones coexisted and diversified rather than a single variant dominating. Unexpectedly, each piglet carried unique populations of bacterial variants, suggesting limited transmission of bacteria between piglets once colonized. Our data show that horizontal gene transfer occurs at very high frequency in vivo and significantly higher than that detectable in vitro.
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影响因子：
5.7
作者：
McCarthy AJ;Witney AA;Lindsay JA
通讯作者：
Lindsay JA
影响因子：
4.2
作者：
McCarthy AJ;Lindsay JA
通讯作者：
Lindsay JA
影响因子：
3.2
作者：
Lindsay, JA;Moore, CE;Hinds, J
通讯作者：
Hinds, J
影响因子：
3.3
作者：
Khanna, T.;Friendship, R.;Weese, J. S.
通讯作者：
Weese, J. S.
DOI：
10.1126/science.1182395
发表时间：
2010-01-22
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Harris SR;Feil EJ;Holden MT;Quail MA;Nickerson EK;Chantratita N;Gardete S;Tavares A;Day N;Lindsay JA;Edgeworth JD;de Lencastre H;Parkhill J;Peacock SJ;Bentley SD
通讯作者：
Bentley SD