Impact of cellular miRNAs on circulating miRNA biomarker signatures.
Impact of cellular miRNAs on circulating miRNA biomarker signatures.
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DOI：
10.1371/journal.pone.0020769
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发表时间：
2011
期刊：
影响因子：
3.7
通讯作者：
Jones KW
中科院分区：
文献类型：
--
作者：
Duttagupta R;Jiang R;Gollub J;Getts RC;Jones KW
通过筛选容易获得的生物标志物，可以改善对复杂的多因素疾病（如癌症）的有效诊断和监测。在人的血液和血浆中发现了以RNA和DNA种类存在的高度稳定的无细胞循环核酸（CNA）。肿瘤相关基因组/表观遗传/转录变化与CNA水平改变之间的相关性是该生物标志物类别作为有前景的临床指标的效用的强预测因子。为了实现这一目标，代表一类长度为19-25 nt的天然存在的小非编码RNA的microRNA（miRNA）已经成为一组重要的标记物，其可以将其特异性表达谱与癌症发展相关联。在这项研究中，我们调查了一些分析前的考虑因素，分离血浆馏分的研究miRNA的生物标志物。我们发现循环miRNA水平的测量经常被不同造血来源的细胞miRNA的不同水平所混淆。为了评估这类细胞来源的相对比例，我们将全血分成血浆及其随后的亚组分。对正常个体的队列中的细胞miRNA特征进行编目，并在校准该干扰类别的信号后探索真实循环标志物的丰度和性别特异性表达。差异表达谱的地图，并在健康男性和女性的子集中研究循环miRNA种类的内在变异性。
Effective diagnosis and surveillance of complex multi-factorial disorders such as cancer can be improved by screening of easily accessible biomarkers. Highly stable cell free Circulating Nucleic Acids (CNA) present as both RNA and DNA species have been discovered in the blood and plasma of humans. Correlations between tumor-associated genomic/epigenetic/transcriptional changes and alterations in CNA levels are strong predictors of the utility of this biomarker class as promising clinical indicators. Towards this goal microRNAs (miRNAs) representing a class of naturally occurring small non-coding RNAs of 19–25 nt in length have emerged as an important set of markers that can associate their specific expression profiles with cancer development. In this study we investigate some of the pre-analytic considerations for isolating plasma fractions for the study of miRNA biomarkers. We find that measurement of circulating miRNA levels are frequently confounded by varying levels of cellular miRNAs of different hematopoietic origins. In order to assess the relative proportions of this cell-derived class, we have fractionated whole blood into plasma and its ensuing sub-fractions. Cellular miRNA signatures in cohorts of normal individuals are catalogued and the abundance and gender specific expression of bona fide circulating markers explored after calibrating the signal for this interfering class. A map of differentially expressed profiles is presented and the intrinsic variability of circulating miRNA species investigated in subsets of healthy males and females.
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影响因子：
14.9
作者：
Griffiths-Jones, S
通讯作者：
Griffiths-Jones, S
DOI：
10.1073/pnas.091062498
发表时间：
2001-04-24
影响因子：
11.1
作者：
Tusher, VG;Tibshirani, R;Chu, G
通讯作者：
Chu, G
影响因子：
6.5
作者：
Lawrie, Charles H.;Gal, Shira;Harris, Adrian L.
通讯作者：
Harris, Adrian L.
Mirbase：MicroRNA基因组学的工具。
影响因子：
14.9
作者：
Griffiths-Jones, Sam;Saini, Harpreet Kaur;van Dongen, Stijn;Enright, Anton J.
通讯作者：
Enright, Anton J.
影响因子：
3.7
作者：
Hunter, Melissa Piper;Ismail, Noura;Zhang, Xiaoli;Aguda, Baltazar D.;Lee, Eun Joo;Yu, Lianbo;Xiao, Tao;Schafer, Jeffrey;Lee, Mei-Ling Ting;Schmittgen, Thomas D.;Nana-Sinkam, S. Patrick;Jarjoura, David;Marsh, Clay B.
通讯作者：
Marsh, Clay B.