Gut microbiota modulation and immunity response induced by Citrobacter freundii strain GC01 in grass carp (Ctenopharyngodon idellus)
Gut microbiota modulation and immunity response induced by Citrobacter freundii strain GC01 in grass carp (Ctenopharyngodon idellus)
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弗氏柠檬酸杆菌GC01菌株在草鱼（Ctenopharyngodon idellus）中诱导的肠道微生物群调节和免疫反应
DOI：
10.1016/j.aquaculture.2020.735015
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发表时间：
2020-05
期刊：
影响因子：
4.5
通讯作者：
Guitang Wang
中科院分区：
文献类型：
--
作者：
Fan Xiong;Lu Qin;Yaotong Hao;Di Zhao;Wenxiang Li;Hong Zou;Ming Li;Shangong Wu;Guitang Wang
弗氏枸橼酸杆菌是鱼类的一种条件致病菌，其对鱼类肠道微生物群和免疫反应的影响尚不清楚。我们用两种不同浓度的这种细菌（实验低和高组，加上对照组）喂养草鱼。采用小肠HE染色法，两个实验组均未发现急性炎症反应的迹象，但高剂量组的小肠杯状细胞数量要高得多。此外，我们还发现C.弗氏杆菌显著降低了两组肠道微生物群的α多样性，改变了β多样性，并显著提高了高水平组肠杆菌目、巴氏杆菌目、奈瑟菌目和柠檬酸菌的相对丰度。脾脏转录组显示，免疫系统相关基因的表达模式在对照组和高剂量组之间存在显著差异。几个免疫相关途径和两个传染病相关途径在高水平组中显著富集。高剂量组补体系统基因c3、c4、c5、c7、c8a、c8和c1s表达上调。总的来说，我们的结果表明C.弗氏杆菌感染并不引起急性肠道炎症，但它改变了肠道菌群的多样性和种群结构，并触发了宿主的固有粘膜免疫系统。两种传染病相关途径的富集也表明高水平组的感染风险更高。这些结果有助于我们了解鱼类机会致病菌对肠道菌群和宿主免疫反应的影响。
The effect ofCitrobacterfreundii, an opportunistic pathogen of fish, on the gut microbiota and immune responses in fish remains unknown. We fed grass carp (Ctenopharyngodon idellus) with two different concentrations of this bacterium (experimental Low and High groups, plus Control group). No signs of acute inflammatory response were found in either of the experimental groups using the intestinal HE staining method, but the number of intestinal goblet cells was much higher in the High group. Furthermore, we found that feedingC. freundiisignificantly decreased the alpha diversity and altered beta diversity of gut microbiota in both groups, and significantly promoted the relative abundance of Enterobacteriales, Pasteurellales, Neisseriales andCitrobacterin the High group. Spleen transcriptome showed that the expression pattern of immune system-related genes varied significantly between the Control group and High group. Several immunity-related pathways and two infectious diseases-related pathways were significantly enriched in the High group. Many complement system genes, includingc3,c4,c5,c7,c8a,c8bandc1s, were also up-regulated in the High group. Overall, our results indicate thatC. freundiidid not trigger acute intestinal inflammation, but it changed the diversity and population structure of gut microbiota, and triggered the host's innate mucosal immune system. The enrichment of two infectious diseases-related pathways also suggested a higher risk of infection in the High group. These results contribute to our understanding of the effects of opportunistic fish pathogens on gut microbiota and host's immune responses.
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影响因子：
4.5
作者：
S. Thanigaivel;S. Vijayakumar;S. Gopinath;A. Mukherjee;N. Chandrasekaran;John Thomas
通讯作者：
S. Thanigaivel;S. Vijayakumar;S. Gopinath;A. Mukherjee;N. Chandrasekaran;John Thomas
影响因子：
0.4
作者：
延子 佐藤;誠久 山根;隆文 川村
通讯作者：
延子 佐藤;誠久 山根;隆文 川村
影响因子：
2.5
作者：
I. Karunasagar;R. Pai
通讯作者：
I. Karunasagar;R. Pai
影响因子：
--
作者：
B. Austin;M. Stobie;P. Robertson
通讯作者：
B. Austin;M. Stobie;P. Robertson
影响因子：
4.4
作者：
Kotob MH;Menanteau-Ledouble S;Kumar G;Abdelzaher M;El-Matbouli M
通讯作者：
El-Matbouli M