Identification of Myocardial Telocytes and Bone Marrow Mesenchymal Stem Cells in Mice
Identification of Myocardial Telocytes and Bone Marrow Mesenchymal Stem Cells in Mice
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小鼠心肌特洛细胞和骨髓间充质干细胞的鉴定
DOI：
10.1177/0963689718796773
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发表时间：
2018-09
影响因子：
3.3
通讯作者：
Junbo Ge
中科院分区：
文献类型：
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作者：
Xin Zhong;Yonghua Zheng;Hua Li;Steve Huang;Junbo Ge
目的：本研究旨在比较心肌端细胞（TC）和骨髓间充质干细胞（MSC）的形态学、免疫表型和细胞因子谱，探讨两种间质细胞的差异。方法：体外培养TCs和MSCs，光镜下观察细胞形态。采用免疫荧光、RT-qPCR和Western blotting检测TC和MSCs中CD 34、c-kit和vimentin的表达水平。收集相关上清液，RayBio小鼠细胞因子抗体芯片检测共49种细胞因子谱。通过ELISA进一步证实了显著不同的细胞因子。结果如下：TCs细胞体积小，突起长，为CD 34 +/c-kit+/vimentin+，而MSCs无终足，为CD 34-/c-kit- /vimentin+。细胞因子谱分析和ELISA检测结果显示，与MSCs相比，TCs培养上清中49种细胞因子中有19种明显升高。此外，19种细胞因子中有9种在TC的上清液中至少增加了2倍。在49种细胞因子中，有30种细胞因子在TCs培养上清中与MSCs培养上清相比无明显变化。结论：使用各种技术，我们发现心肌TC和MSC在细胞结构和细胞因子谱方面存在显著差异。
Objectives: The aim of this study was to compare the morphology, immune phenotype, and cytokine profiles between myocardial telocytes (TCs) and bone marrow mesenchymal stem cells (MSCs), and explore the difference between those two types of interstitial cells. Methods: TCs and MSCs were cultured in vitro and cell morphology was observed with a light microscope. The expression levels of CD34, c-kit, and vimentin were detected by immunofluorescence, RT-qPCR, and Western blotting in both TCs and MSCs. The related supernatant was collected and total of 49 cytokine profiles were detected by RayBio Mice Cytokine Antibody Array. Significantly different cytokines were further confirmed by ELISA. Results: TCs have small cellular body and very long prolongations and they were CD34+/c-kit+/vimentin+, whereas MSCs have no telopodes and they were CD34–/c-kit– /vimentin+. Cytokine profile analysis and ELISA showed that 19 of 49 cytokines were increased dramatically in the supernatant of TCs compared with those of MSCs. Moreover, 9 of 19 cytokines were increased 2-fold at least in the supernatant of TCs compared with those of MSCs. Of 49 cytokines, 30 exhibited no significant changes in the supernatant of TCs compared with those of MSCs. Conclusions: Using various technologies, we identified that myocardial TCs and MSCs are significantly different in terms of cell structure and cytokine profiles.
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影响因子：
2.3
作者：
Cretoiu, Sanda Maria;Radu, Beatrice Mihaela;Banciu, Adela;Banciu, Daniel Dumitru;Cretoiu, Dragos;Ceafalan, Laura Cristina;Popescu, Laurentiu Mircea
通讯作者：
Popescu, Laurentiu Mircea
影响因子：
7.6
作者：
Manabu Takewaki;M. Kajiya;K. Takeda;Shinji Sasaki;Souta Motoike;Nao Komatsu;S. Matsuda;K. Ouhara;N. Mizuno;T. Fujita;H. Kurihara
通讯作者：
Manabu Takewaki;M. Kajiya;K. Takeda;Shinji Sasaki;Souta Motoike;Nao Komatsu;S. Matsuda;K. Ouhara;N. Mizuno;T. Fujita;H. Kurihara
影响因子：
3.3
作者：
Zhang H;Caudle Y;Wheeler C;Zhou Y;Stuart C;Yao B;Yin D
通讯作者：
Yin D
影响因子：
--
作者：
Cretoiu, Sanda M;Popescu, Laurentiu M
通讯作者：
Popescu, Laurentiu M
影响因子：
7.5
作者：
Kiyotaka Okada;Kotarou Kojima;K. Okumoto;N. Kawao;O. Matsuo;H. Kaji
通讯作者：
Kiyotaka Okada;Kotarou Kojima;K. Okumoto;N. Kawao;O. Matsuo;H. Kaji