Isolated human uterine telocytes: immunocytochemistry and electrophysiology of T-type calcium channels.
Isolated human uterine telocytes: immunocytochemistry and electrophysiology of T-type calcium channels.
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DOI：
10.1007/s00418-014-1268-0
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发表时间：
2015-01
影响因子：
2.3
通讯作者：
Popescu, Laurentiu Mircea
中科院分区：
文献类型：
--
作者：
Cretoiu, Sanda Maria;Radu, Beatrice Mihaela;Banciu, Adela;Banciu, Daniel Dumitru;Cretoiu, Dragos;Ceafalan, Laura Cristina;Popescu, Laurentiu Mircea
最近，特洛细胞（TC）被描述为许多器官（包括子宫肌层）间质空间中的一种新细胞类型。 TC 是具有非常长、独特的延伸的细胞，称为端足 (Tps)。研究表明，TC 在细胞间信号传导以及形态发生中发挥着重要作用，特别是在形态发生生物电信号传导中。然而，TC 质膜的离子通道和泵的存在和活性尚未得到探索。在这里，我们结合使用体外免疫荧光和膜片钳技术来表征 TC 中的 T 型钙通道。子宫肌层 TC 在细胞培养物（非妊娠和妊娠子宫肌层）中被鉴定为具有很长 Tps 且 CD34 和血小板衍生生长因子受体 α 呈阳性的细胞。对 T 型（瞬时）钙通道亚家族 CaV3.1 和 CaV3.2 存在的免疫荧光分析揭示了这些离子通道在细胞体上的表达以及非妊娠和妊娠子宫肌层 TC 的 Tps。来自非妊娠子宫肌层的 TC 中的表达强度较低，CaV3.2 仅限于细胞体，而 CaV3.1 在细胞体和 Tps 中均表达。此外，通过应用短暂的斜坡去极化方案也证实了非妊娠子宫肌层 TC 中 T 型钙通道的存在。总之，我们的结果表明，T 型钙通道存在于来自人类子宫肌层的 TC 中，并且可以参与内源性生物电信号的产生，负责在怀孕和分娩期间调节周围细胞的行为。
Recently, telocytes (TCs) were described as a new cell type in the interstitial space of many organs, including myometrium. TCs are cells with very long, distinctive extensions named telopodes (Tps). It is suggested that TCs play a major role in intercellular signaling, as well as in morphogenesis, especially in morphogenetic bioelectrical signaling. However, TC plasma membrane is yet unexplored regarding the presence and activity of ion channels and pumps. Here, we used a combination of in vitro immunofluorescence and patch-clamp technique to characterize T-type calcium channels in TCs. Myometrial TCs were identified in cell culture (non-pregnant and pregnant myometrium) as cells having very long Tps and which were positive for CD34 and platelet-derived growth factor receptor-α. Immunofluorescence analysis of the subfamily of T-type (transient) calcium channels CaV3.1 and CaV3.2 presence revealed the expression of these ion channels on the cell body and Tps of non-pregnant and pregnant myometrium TCs. The expression in TCs from the non-pregnant myometrium is less intense, being confined to the cell body for CaV3.2, while CaV3.1 was expressed both on the cell body and in Tps. Moreover, the presence of T-type calcium channels in TCs from non-pregnant myometrium is also confirmed by applying brief ramp depolarization protocols. In conclusion, our results show that T-type calcium channels are present in TCs from human myometrium and could participate in the generation of endogenous bioelectric signals responsible for the regulation of the surrounding cell behavior, during pregnancy and labor.
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影响因子：
5.3
作者：
Chen X;Zheng Y;Manole CG;Wang X;Wang Q
通讯作者：
Wang Q
影响因子：
5.3
作者：
Díaz-Flores L;Gutiérrez R;Sáez FJ;Díaz-Flores L Jr;Madrid JF
通讯作者：
Madrid JF
影响因子：
5.3
作者：
Luesma MJ;Gherghiceanu M;Popescu LM
通讯作者：
Popescu LM
影响因子：
5.3
作者：
Ceafalan L;Gherghiceanu M;Popescu LM;Simionescu O
通讯作者：
Simionescu O
DOI：
10.1530/rep-12-0369
发表时间：
2013-04
期刊：
Reproduction (Cambridge, England)
影响因子：
--
作者：
Cretoiu SM;Cretoiu D;Marin A;Radu BM;Popescu LM
通讯作者：
Popescu LM