Transcriptome and expression profile analysis of highly resistant and susceptible banana roots challenged with Fusarium oxysporum f. sp. cubense tropical race 4.
Transcriptome and expression profile analysis of highly resistant and susceptible banana roots challenged with Fusarium oxysporum f. sp. cubense tropical race 4.
复制标题
DOI：
10.1371/journal.pone.0073945
复制
发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Li HP
中科院分区：
文献类型：
--
作者：
Bai TT;Xie WB;Zhou PP;Wu ZL;Xiao WC;Zhou L;Sun J;Ruan XL;Li HP
香蕉枯萎病是由香蕉枯萎病菌引起的一种病害。cubense 4（Foc 4）是世界上最具破坏性的病害之一。在温室和田间试验中，卡文迪什品种‘粤油抗1号’与感病品种‘巴西’相比，病情严重程度和发病率显著降低。先前利用从头测序技术研究了中抗品种‘农科1号’香蕉的防御机制，但没有研究高抗品种‘粤优抗1号’香蕉的防御机制。为了深入了解香蕉对Foc 4的抗性机制，利用Illumina Solexa测序技术对“粤优抗1号”和“巴西”进行转录组测序，分析了Foc 4感染后0.5、1、3、5和10 d基因表达谱的变化。结果表明，Foc 4处理后，“粤优康1号”的转录重编程发生率高于“巴西”，尤其是在前3个时间点，表明“粤优康1号”对Foc 4感染的防御反应比“巴西”快得多。分析比较了“植物-病原菌互作”和“植物激素信号转导”途径相关基因在两个品种间的表达模式。与CEBiP、BAK 1、NB-LRR蛋白、PR蛋白、转录因子和细胞壁木质化相关的防御基因在“粤优抗1号”中的表达量高于“巴西”，表明这些基因在香蕉抗Foc 4侵染中起重要作用。而与过敏反应（hypersensitivity reaction，HR）和衰老相关的基因在“巴西”中表达上调，而在“粤优康1号”中表达下调，提示HR和衰老可能与Foc 4感染有关。另外，高抗品种‘粤优抗1号’的抗病机制与中抗品种‘农科1号’不同。这些结果解释了高抗品种的抗性，并为理解香蕉与Foc 4之间的亲和和不亲和相互作用提供了更多的见解。
Banana wilt disease, caused by the fungal pathogen Fusarium oxysporum f. sp. cubense 4 (Foc4), is regarded as one of the most devastating diseases worldwide. Cavendish cultivar ‘Yueyoukang 1’ was shown to have significantly lower disease severity and incidence compared with susceptible cultivar ‘Brazilian’ in greenhouse and field trials. De novo sequencing technology was previously performed to investigate defense mechanism in middle resistant ‘Nongke No 1’ banana, but not in highly resistant cultivar ‘Yueyoukang 1’. To gain more insights into the resistance mechanism in banana against Foc4, Illumina Solexa sequencing technology was utilized to perform transcriptome sequencing of ‘Yueyoukang 1’ and ‘Brazilian’ and characterize gene expression profile changes in the both two cultivars at days 0.5, 1, 3, 5 and 10 after infection with Foc4. The results showed that more massive transcriptional reprogramming occurs due to Foc4 treatment in ‘Yueyoukang 1’ than ‘Brazilian’, especially at the first three time points, which suggested that ‘Yueyoukang 1’ had much faster defense response against Foc4 infection than ‘Brazilian’. Expression patterns of genes involved in ‘Plant-pathogen interaction’ and ‘Plant hormone signal transduction’ pathways were analyzed and compared between the two cultivars. Defense genes associated with CEBiP, BAK1, NB-LRR proteins, PR proteins, transcription factor and cell wall lignification were expressed stronger in ‘Yueyoukang 1’ than ‘Brazilian’, indicating that these genes play important roles in banana against Foc4 infection. However, genes related to hypersensitive reaction (HR) and senescence were up-regulated in ‘Brazilian’ but down-regulated in ‘Yueyoukang 1’, which suggested that HR and senescence may contribute to Foc4 infection. In addition, the resistance mechanism in highly resistant ‘Yueyoukang 1’ was found to differ from that in middle resistant ‘Nongke No 1’ banana. These results explain the resistance in the highly resistant cultivar and provide more insights in understanding the compatible and incompatible interactions between banana and Foc4.
登录
查看更多内容
影响因子：
3.6
作者：
Hegedus, Zoltan;Zakrzewska, Anna;Meijer, Annemarie H.
通讯作者：
Meijer, Annemarie H.
影响因子：
4.5
作者：
Hwang, SC;Ko, WH
通讯作者：
Ko, WH
影响因子：
11.6
作者：
Balagué, C;Lin, BQ;Roby, D
通讯作者：
Roby, D
影响因子：
64.8
作者：
Asai, T;Tena, G;Sheen, J
通讯作者：
Sheen, J
DOI：
10.1073/pnas.0508882103
发表时间：
2006-07-18
影响因子：
11.1
作者：
Kaku, Hanae;Nishizawa, Yoko;Shibuya, Naoto
通讯作者：
Shibuya, Naoto