StTCP15 regulates potato tuber sprouting by modulating the dynamic balance between abscisic acid and gibberellic acid.
StTCP15 regulates potato tuber sprouting by modulating the dynamic balance between abscisic acid and gibberellic acid.
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DOI：
10.3389/fpls.2022.1009552
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发表时间：
2022
影响因子：
5.6
通讯作者：
Si, Huaijun
中科院分区：
文献类型：
--
作者：
Wang, Kaitong;Zhang, Ning;Fu, Xue;Zhang, Huanhuan;Liu, Shengyan;Pu, Xue;Wang, Xiao;Si, Huaijun
马铃薯生命周期的主要阶段是块茎休眠和发芽，然而，控制这些过程的机制仍然知之甚少。TCP（Theosinte分支I，Cycloidea，proliferationcellfactors1和2）转录因子在植物生长和休眠相关的发育过程中起着关键作用。前期研究表明TCP转录因子StTCP15具有促进休眠的功能。为了阐明StTCP 15基因的功能，从马铃薯栽培品种"Desiree"中克隆了该基因，其编码由414个氨基酸组成的多肽，并且主要存在于细胞核中。StTCP15过表达株系的马铃薯块茎提前发芽，而StTCP15下调表达株系的马铃薯块茎延迟发芽。此外，还发现StTCP 15过表达株系与野生型相比极显著地降低了脱落酸（ABA）/赤霉酸（GA3）的比值，同时超氧化物歧化酶活性降低，过氧化物酶和过氧化氢酶活性升高。而StTCP 15基因表达下调的细胞株则相反。利用酵母双杂交技术筛选到3个相互作用蛋白StSnRK1、StF-Box和StGID1，并通过双分子荧光互补技术和分裂荧光素酶技术进行验证，表明StTCP 15可以通过影响ABA和GA3信号通路来调控马铃薯块茎休眠和发芽。这些结果表明，StTCP 15通过影响ABA和GA3之间的动态平衡来调节马铃薯块茎休眠和发芽。本研究结果为StTCP 15调控马铃薯块茎休眠和发芽的分子机制提供了一定的信息。
The major stages of the potato life cycle are tuber dormancy and sprouting, however, there is still known very little of the mechanisms that control these processes. TCP (Theosinte branch I, Cycloidea, proliferationcell factors 1 and 2) transcription factors play a key role in plant growth and dormancy related developmental processes. Previous researches demonstrated that TCP transcription factor StTCP15 had a function in the promotion of dormancy. To elucidate the function of StTCP15 gene, it was cloned from potato cultivar “Desiree,” which encodes a polypeptide consisting of 414 amino acids and is mainly found in the nucleus. The potato tubers of StTCP15 overexpression lines sprouted in advance, while the potato tubers of StTCP15 down-regulated expression lines showed delayed sprouting. In addition, it was also found that overexpression lines of StTCP15 extremely significantly reduced the ratio of abscisic acid (ABA)/gibberellic acid (GA3), while the superoxide dismutase activity decreased, and the activity of peroxidase and catalase increased compared with the wild type. The opposite result was found in the down-regulated expression lines of StTCP15 gene. Three interacting proteins, StSnRK1, StF-Box and StGID1, were screened by Yeast two-hybrid, and verified by Bimolecular Fluorescence Complementation and Split-luciferase, indicating that StTCP15 could affect ABA and GA3 signaling pathways to regulate potato tuber dormancy and sprouting. Together, these results demonstrated that StTCP15 regulated potato tuber dormancy and sprouting by affecting the dynamic balance between ABA and GA3. The result could provide some information on the molecular mechanism of StTCP15 regulating potato tuber dormancy and sprouting.
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