Development and Application of InDel Markers Linked to Fruit-Shape and Peel-Colour Genes in Wax Gourd.
Development and Application of InDel Markers Linked to Fruit-Shape and Peel-Colour Genes in Wax Gourd.
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冬瓜具有很高的营养价值和经济价值，在许多国家都有种植。虽然控制冬瓜果实形状和果皮颜色的基因已经被报道，但与这些基因相关联的Indel标记仍然没有开发出来。本研究从测序数据中筛选出与果形(Bch02G016830)和果皮颜色(Bch05G003950)基因连锁的Indel标记。我们利用120个自交系、536个隔离群体和4个商业杂交种对Indel标记的有效性和应用价值进行了评估。在120份自交系中，9对果形Indel标记(GX1~GX9)的准确率为84.16~91.66%。在120份自交系中，皮色基因上游约3.0Mb和下游约3.0Mb的27对皮色标记(PS1~PS27)的准确率为100%，皮色基因上下游约3.0~20Mb的6对皮色标记(PS28~PS33)的准确率为55.83~90%。用GX1、GX2、PS13和Ps14对4个商品杂交种进行纯度测定，结果与田间测定结果高度一致。本研究结果为冬瓜杂交种的遗传连锁图谱构建、分子标记辅助选择育种和纯度鉴定提供了重要信息。
The wax gourd is commonly grown in many countries because of its high nutritional and economic value. While the genes for the fruit shape and peel colour of wax gourd have been reported, the InDel markers linked to these genes remain undeveloped. In this study, the InDel markers linked to fruit-shape (Bch02G016830) and peel-colour (Bch05G003950) genes were developed from resequenced data. We used 120 inbred lines, 536 isolated populations, and 4 commercial hybrids to evaluate the validity and application value of the InDel markers. The accuracy rates of nine pairs of fruit-shape InDel markers (GX1-GX9) were 84.16–91.66% in 120 inbred lines. The accuracy rates of 27 pairs of peel-colour InDel markers (PS1-PS27) within approximately 3.0 Mb upstream and 3.0 Mb downstream of the peel-colour gene were 100% and those of 6 pairs of peel-colour InDel markers (PS28-PS33) within 3.0–20 Mb upstream and downstream of the peel-colour gene were 55.83–90% in 120 inbred lines. The purity of four commercial hybrids determined using GX1, GX2, PS13, and PS14 was highly consistent with the field results for purity determination. Our results provide important information for genetic linkage map construction, molecular-marker-assisted selective breeding, and purity determination of wax gourd hybrids.
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