Molecular evidence that most but not all carcinosarcomas of the uterus are combination tumors.
Molecular evidence that most but not all carcinosarcomas of the uterus are combination tumors.
复制标题
分子证据表明大多数但并非全部子宫癌肉瘤都是组合肿瘤。
作者：
H. Wada;T. Enomoto;M. Fujita;K. Yoshino;R. Nakashima;H. Kurachi;T. Haba;Ken'ichi Wakasa;K. Shroyer;M. Tsujimoto;T. Hongyo;Taisei Nomura;Y. Murata
癌肉瘤的发病机制至今仍有争议。本研究应用分子生物学技术探讨子宫癌肉瘤的发病机制。针对恶性上皮和间质成分中人雄激素受体（HUMARA）外显子1的一部分，分析了X染色体失活的模式。p53和K-ras突变的存在也进行了分析。石蜡包埋，福尔马林固定的组织的H& E染色切片进行显微解剖，以获得上皮和非上皮病变从25个癌细胞瘤，DNA提取蛋白酶K消化。用甲基化敏感性限制性内切酶（HhaI或HpaII）处理后，使用靶向HUMARA基因座的巢式引物进行PCR扩增。p53基因和K-ras基因突变分别在8例（32%）和6例（24%）肿瘤中发现。X染色体失活的模式在三个癌性肉瘤的癌性和肉瘤性成分之间是不同的，表明这三个肿瘤代表碰撞肿瘤。相比之下，X染色体失活，K-ras序列，和p53序列的模式是相同的癌和肉瘤的组成部分，在21个癌肉瘤，这表明这21个肿瘤代表组合肿瘤。1例病例产生了不确定的结果，无法确定其是否代表碰撞或联合肿瘤。这些观察结果表明，虽然大多数癌细胞瘤是混合性肿瘤，但也有一些发展为碰撞性肿瘤。应用分子标记物对癌肉瘤的组织发生进行个体化检测，有助于预测个体化病例的预后，指导临床治疗。
The pathogenesis of carcinosarcoma is still a subject of controversy. In the present study, molecular techniques were applied to determine the pathogenesis of uterine carcinosarcomas. The patterns of chromosome X inactivation were analyzed, targeting a portion of exon 1 of the human androgen receptor (HUMARA) in malignant epithelial and mesenchymal components. The presence of p53 and K-ras mutations were also analyzed. H&E-stained sections of paraffin-embedded, formalin-fixed tissues were microdissected to obtain both epithelial and nonepithelial lesions from 25 carcinosarcomas, and DNAs were extracted by proteinase K digestion. Following treatment with methylation-sensitive restriction endonuclease (HhaI or HpaII), PCR amplification was performed using nested primers targeted to the HUMARA locus. Mutations in the p53 gene and K-ras gene were found in eight (32%) and six (24%) tumors, respectively. The patterns of chromosome X inactivation were different between the carcinomatous and sarcomatous components of three carcinosarcomas, indicating that these three tumors represent collision tumors. By contrast, the patterns of chromosome X inactivation, K-ras sequence, and p53 sequence were identical in both carcinomatous and sarcomatous components in 21 carcinosarcomas, indicating that these 21 tumors represent combination tumors. One case produced equivocal results that precluded determination of whether it represented a collision or combination tumor. These observations show that although most carcinosarcomas are combination tumors, some develop as collision tumors. The determination of histogenesis in individual cases of carcinosarcoma using molecular markers may be worthwhile, because the result could help predict the prognosis of individual cases and help guide clinical management.
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影响因子：
11.2
作者：
Joyce Bos
通讯作者：
Joyce Bos
K-ras 在人类子宫癌前和恶性上皮病变中的激活。
影响因子：
11.2
作者：
Enomoto,T;Inoue,M;Perantoni,AO;Buzard,GS;Miki,H;Tanizawa,O;Rice,JM
通讯作者：
Rice,JM
非随机 X 染色体失活的聚合酶链反应测定可识别单克隆子宫内膜癌和癌前病变。
DOI：
--
发表时间：
1995
期刊：
The American journal of pathology.
影响因子：
--
作者：
Mutter,GL;Chaponot,ML;Fletcher,JA
通讯作者：
Fletcher,JA
DOI：
10.1073/pnas.86.1.327
发表时间：
1989-01-01
影响因子：
11.1
作者：
TILLEY, WD;MARCELLI, M;MCPHAUL, MJ
通讯作者：
MCPHAUL, MJ
影响因子：
5.6
作者：
Thompson, L;Chang, B;Barsky, SH
通讯作者：
Barsky, SH