Trojan horselike T6SS effector TepC mediates both interference competition and exploitative competition
Trojan horselike T6SS effector TepC mediates both interference competition and exploitative competition
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DOI：
10.1093/ismejo/wrad028
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发表时间：
2024-01-10
期刊：
影响因子：
11
通讯作者：
Shen, Xihui
中科院分区：
文献类型：
--
作者：
Song, Li;Xu, Lei;Wu, Tong;Shi, Zhenkun;Kareem, Hafiz Abdul;Wang, Zhuo;Dai, Qingyun;Guo, Chenghao;Pan, Junfeng;Yang, Mingming;Wei, Xiaomeng;Wang, Yao;Wei, Gehong;Shen, Xihui
VI型分泌系统（T6SS）是一种细菌武器，能够输送抗菌效应物杀死竞争细胞进行干扰竞争，并分泌金属离子清除效应物以获得必需的微量营养素以进行开发竞争。然而，目前还没有报道可以调解干扰竞争和利用竞争的T6SS效应器。在这项研究中，我们在假结核耶尔森氏菌中发现了一种独特的 T6SS-1 效应子，名为 TepC，它在微生物群落中发挥着多种作用。首先，分泌的 TepC 作为蛋白质铁载体，与铁结合并介导剥削性竞争。此外，我们发现 TepC 具有 DNase 活性，这使其具有接触依赖性和接触非依赖性干扰竞争能力。在铁有限的情况下，负载铁的 TepC 被表达外膜受体 TdsR 的靶细胞吸收。对于编码同源免疫蛋白 TipC 的亲属细胞，TepC 促进铁的获取，并中和其毒性作用。另一方面，缺乏 TipC 的非亲缘细胞会被诱导摄取 TepC，并被其 DNase 活性杀死。因此，我们发现了一种T6SS效应器TepC，它的功能就像“特洛伊木马”一样，通过与铁离子结合，为亲属细胞提供宝贵的资源，同时惩罚不生产公共物品的作弊者。这种由双功能 T6SS 效应器介导的诱杀机制可能为维持微生物群落稳定性的分子机制提供新的见解。
The type VI secretion system (T6SS) is a bacterial weapon capable of delivering antibacterial effectors to kill competing cells for interference competition, as well as secreting metal ion scavenging effectors to acquire essential micronutrients for exploitation competition. However, no T6SS effectors that can mediate both interference competition and exploitation competition have been reported. In this study, we identified a unique T6SS-1 effector in Yersinia pseudotuberculosis named TepC, which plays versatile roles in microbial communities. First, secreted TepC acts as a proteinaceous siderophore that binds to iron and mediates exploitative competition. Additionally, we discovered that TepC has DNase activity, which gives it both contact-dependent and contact-independent interference competition abilities. In conditions where iron is limited, the iron-loaded TepC is taken up by target cells expressing the outer membrane receptor TdsR. For kin cells encoding the cognate immunity protein TipC, TepC facilitates iron acquisition, and its toxic effects are neutralized. On the other hand, nonkin cells lacking TipC are enticed to uptake TepC and are killed by its DNase activity. Therefore, we have uncovered a T6SS effector, TepC, that functions like a “Trojan horse” by binding to iron ions to provide a valuable resource to kin cells, whereas punishing cheaters that do not produce public goods. This lure-to-kill mechanism, mediated by a bifunctional T6SS effector, may offer new insights into the molecular mechanisms that maintain stability in microbial communities.
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影响因子：
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影响因子：
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