Multimethodological approach to identification of glycoproteins from the proteome of Francisella tularensis, an intracellular microorganism.
Multimethodological approach to identification of glycoproteins from the proteome of Francisella tularensis, an intracellular microorganism.
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DOI：
10.1021/pr9011602
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发表时间：
2010-04-05
影响因子：
4.4
通讯作者：
Stulik, Jiri
中科院分区：
文献类型：
--
作者：
Balonova, Lucie;Hernychova, Lenka;Mann, Benjamin F.;Link, Marek;Bilkova, Zuzana;Novotny, Milos V.;Stulik, Jiri
看来病原细菌中发现的大多数糖蛋白都与毒力有关。尽管最近发现了新的毒力因子，但土拉弗朗西斯菌的毒力机制仍知之甚少。尽管它很重要，但有关该细菌中蛋白质糖基化及其与细菌毒力的潜在联系的问题尚未得到解答。在本研究中，利用自下而上的蛋白质组学方法，通过将碳水化合物特异性检测和凝集素亲和力与高灵敏质谱法相结合，对几种假定的土拉弗朗西斯菌糖蛋白进行了表征。最近发现可能被糖基化的蛋白质 PilA 以及被指定为新毒力因子的其他蛋白质均属于本研究中鉴定的蛋白质。报告的数据编制了潜在糖蛋白列表，可作为进一步定义聚糖修饰的蛋白质的起飞平台，有助于更好地了解蛋白质糖基化在土拉弗朗西斯菌致病性中的功能。
It appears that most glycoproteins found in pathogenic bacteria are associated with virulence. Despite the recent identification of novel virulence factors, the mechanisms of virulence in Francisella tularensis are poorly understood. In spite of its importance, questions about glycosylation of proteins in this bacterium and its potential connection with bacterial virulence have not been answered yet. In the present study, several putative Francisella tularensis glycoproteins were characterized through the combination of carbohydrate-specific detection and lectin affinity with highly sensitive mass spectrometry utilizing the bottom-up proteomic approach. The protein PilA that was recently found as being possibly glycosylated, as well as other proteins with designation as novel factors of virulence, were among the proteins identified in this study. The reported data compile the list of potential glycoproteins that may serve as a take-off platform for a further definition of proteins modified by glycans, faciliting a better understanding of the function of protein glycosylation in pathogenicity of Francisella tularensis.
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影响因子：
3.2
作者：
Nano, FE;Zhang, N;Elkins, KL
通讯作者：
Elkins, KL
影响因子：
3.2
作者：
Dobos, KM;Khoo, KH;Belisle, JT
通讯作者：
Belisle, JT
影响因子：
4.4
作者：
Gonzalez-Zamnorano, Margarita;Mendoza-Hernandez, Guillermo;Espitia, Clara
通讯作者：
Espitia, Clara
DOI：
10.1111/j.1432-1033.1989.tb14915.x
发表时间：
1989-07-15
期刊：
EUROPEAN JOURNAL OF BIOCHEMISTRY
影响因子：
--
作者：
DAGOSTARO, G;BENDIAK, B;TROPAK, M
通讯作者：
TROPAK, M
影响因子：
3.2
作者：
HRABAK, EM;WILLIS, DK
通讯作者：
WILLIS, DK