3-Deoxysappanchalcone Promotes Proliferation of Human Hair Follicle Dermal Papilla Cells and Hair Growth in C57BL/6 Mice by Modulating WNT/β-Catenin and STAT Signaling.
3-Deoxysappanchalcone Promotes Proliferation of Human Hair Follicle Dermal Papilla Cells and Hair Growth in C57BL/6 Mice by Modulating WNT/β-Catenin and STAT Signaling.
复制标题
DOI：
10.4062/biomolther.2016.183
复制
发表时间：
2016-11-01
影响因子：
3.7
通讯作者：
Choi BY
中科院分区：
文献类型：
--
作者：
Kim YE;Choi HC;Lee IC;Yuk DY;Lee H;Choi BY
3-3-DSC具有抗过敏、抗病毒、抗炎和抗氧化等活性。本研究观察了3-DSC对人毛囊毛乳头细胞（HDPCs）增殖和小鼠毛发生长的影响。采用实时细胞分析系统、荧光素酶检测、Western blot和实时聚合酶链反应（PCR）检测HDPCs对3-DSC处理的反应。还检查了3-DSC对C57 BL/6小鼠毛发生长的影响。3-DSC促进HDPC的增殖，类似于Janus激活激酶（JAK）抑制剂托法替尼。3-DSC促进β-catenin的磷酸化和T细胞因子的转录激活。此外，3-DSC增强白细胞介素-6（IL-6）诱导的磷酸化和随后的信号转导和转录激活因子-3（STAT 3）的反式激活，从而增加细胞周期蛋白依赖性激酶-4（Cdk 4）、成纤维细胞生长因子（FGF）和血管内皮生长因子（VEGF）的表达。相反，3-DSC减弱了HDPC中STAT 6 mRNA的表达和IL 4诱导的STAT 6磷酸化。最后，我们观察到局部应用3-DSC促进C57 BL/6小鼠毛发生长的生长期。3-DSC可能通过调节WNT/β-catenin和STAT信号诱导毛囊毛乳头细胞增殖来刺激毛发生长。
3-Deoxysappanchalcone (3-DSC) has been reported to possess anti-allergic, antiviral, anti-inflammatory and antioxidant activities. In the present study, we investigated the effects of 3-DSC on the proliferation of human hair follicle dermal papilla cells (HDPCs) and mouse hair growth in vivo. A real-time cell analyzer system, luciferase assay, Western blot and real-time polymerase chain reaction (PCR) were employed to measure the biochemical changes occurring in HDPCs in response to 3-DSC treatment. The effect of 3-DSC on hair growth in C57BL/6 mice was also examined. 3-DSC promoted the proliferation of HDPCs, similar to Tofacitinib, an inhibitor of janus-activated kinase (JAK). 3-DSC promoted phosphorylation of β-catenin and transcriptional activation of the T-cell factor. In addition, 3-DSC potentiated interleukin-6 (IL-6)-induced phosphorylation and subsequent transactivation of signal transducer and activator of transcription-3 (STAT3), thereby increasing the expression of cyclin-dependent kinase-4 (Cdk4), fibroblast growth factor (FGF) and vascular endothelial growth factor (VEGF). On the contrary, 3-DSC attenuated STAT6 mRNA expression and IL4-induced STAT6 phosphorylation in HDPCs. Finally, we observed that topical application of 3-DSC promoted the anagen phase of hair growth in C57BL/6 mice. 3-DSC stimulates hair growth possibly by inducing proliferation of follicular dermal papilla cells via modulation of WNT/β-catenin and STAT signaling.
登录
查看更多内容
影响因子：
82.9
作者：
通讯作者：
--
影响因子：
2.7
作者：
Liu, Ai-Lin;Shu, Shi-Hui;Du, Guan-Hua
通讯作者：
Du, Guan-Hua
影响因子：
4.8
作者：
Kotanides, H;Reich, NC
通讯作者：
Reich, NC
DOI：
10.1073/pnas.0407114101
发表时间：
2004-11-09
影响因子：
11.1
作者：
Lin, KK;Chudova, D;Andersen, B
通讯作者：
Andersen, B
影响因子：
64.5
作者：
Festa E;Fretz J;Berry R;Schmidt B;Rodeheffer M;Horowitz M;Horsley V
通讯作者：
Horsley V