Hydrogel containing nanoparticle-stabilized liposomes for topical antimicrobial delivery.
Hydrogel containing nanoparticle-stabilized liposomes for topical antimicrobial delivery.
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DOI：
10.1021/nn500110a
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发表时间：
2014-03-25
期刊：
影响因子：
17.1
通讯作者：
Zhang, Liangfang
中科院分区：
文献类型：
--
作者：
Gao, Weiwei;Vecchio, Drew;Li, Jieming;Zhu, Jingying;Zhang, Qiangzhe;Fu, Victoria;Li, Jiayang;Thamphiwatana, Soracha;Lu, Diannan;Zhang, Liangfang
将小的带电纳米颗粒吸附到脂质体的外表面上已经成为一种有效的策略，以在“看到”靶细菌之前稳定脂质体以防止融合，但允许它们在到达感染部位时与细菌融合。因此，纳米颗粒稳定的脂质体已成为治疗各种细菌感染的新兴药物递送平台。为了促进该平台在临床试验和应用中的转化，我们将纳米颗粒稳定的脂质体与水凝胶技术相结合，以实现更有效和持续的局部药物递送。水凝胶制剂不仅保留了纳米颗粒稳定的脂质体的结构完整性，而且还允许可控的粘弹性和可调的脂质体释放速率。使用金黄色葡萄球菌细菌作为模型病原体，我们证明了水凝胶制剂可以有效地将纳米颗粒稳定的脂质体释放到细菌培养物中，随后以pH依赖性方式与细菌膜融合。当局部施用到小鼠皮肤上时，水凝胶制剂在7天治疗内不产生任何可观察到的皮肤毒性。总的来说，含有纳米颗粒稳定的脂质体的水凝胶对于针对各种微生物感染的局部应用具有很大的希望。
Adsorbing small charged nanoparticles onto the outer surfaces of liposomes has become an effective strategy to stabilize liposomes against fusion prior to “seeing” target bacteria, yet allow them to fuse with the bacteria upon arrival at the infection sites. As a result, nanoparticle-stabilized liposomes have become an emerging drug delivery platform for treatment of various bacterial infections. To facilitate the translation of this platform for clinical tests and uses, herein we integrate nanoparticle-stabilized liposomes with hydrogel technology for more effective and sustained topical drug delivery. The hydrogel formulation not only preserves the structural integrity of the nanoparticle-stabilized liposomes, but also allows for controllable viscoeleasticity and tunable liposome release rate. Using Staphylococcus aureus bacteria as a model pathogen, we demonstrate that the hydrogel formulation can effectively release nanoparticle-stabilized liposomes to the bacterial culture, which subsequently fuse with bacterial membrane in a pH-dependent manner. When topically applied onto mouse skin, the hydrogel formulation does not generate any observable skin toxicity within a 7-day treatment. Collectively, the hydrogel containing nanoparticle-stabilized liposomes hold great promise for topical applications against various microbial infections.
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DOI：
10.1016/j.saa.2005.04.048
发表时间：
2006-01-01
影响因子：
4.4
作者：
Aryal, S;Remant, BKC;Kim, HY
通讯作者：
Kim, HY
影响因子：
1.5
作者：
Negredo, Eugenia;Puig, Jordi;Clotet, Bonaventura
通讯作者：
Clotet, Bonaventura
影响因子：
3.8
作者：
HIGUCHI, T
通讯作者：
HIGUCHI, T
影响因子：
6.2
作者：
Popescu, Maria-Teodora;Mourtas, Spyridon;Tsitsilianis, Constantinos
通讯作者：
Tsitsilianis, Constantinos
DOI：
10.1073/pnas.0602766103
发表时间：
2006-10-24
影响因子：
11.1
作者：
Haluska, Christopher K.;Riske, Karin A.;Dimova, Rumiana
通讯作者：
Dimova, Rumiana