Characteristics and differences of gut microbiota in patients with different Traditional Chinese Medicine Syndromes of Colorectal Cancer and normal population.
Characteristics and differences of gut microbiota in patients with different Traditional Chinese Medicine Syndromes of Colorectal Cancer and normal population.
复制标题
DOI：
10.7150/jca.50318
复制
发表时间：
2020
影响因子：
3.9
通讯作者：
Ruan S
中科院分区：
文献类型：
--
作者：
Wang P;Ding S;Sun L;Feng Y;Guo K;Zhu Y;Huang D;Ruan S
结直肠癌（CRC）被认为与肠道微生物的改变密切相关。本研究的目的是探讨肠道微生物群的分布，以区分正气亏虚（ZQKX）和邪毒用盛（XDYS）两种疾病综合征之间的微生物群失调。首先，2019年2月至2019年6月，符合既定纳入和排除标准的在浙江省中医院肿瘤科就诊的CRC患者被纳入本前瞻性研究。收集健康志愿者和患有ZQKX或XDYS综合征的CRC患者的新鲜粪便标本后，16S rRNA基因扩增和测序可用于鉴定各组间肠道微生物群的多样性和丰度。结果表明，普通对照组的微生物群组成优于实验组。在门水平上，在健康志愿者中观察到拟杆菌丰度显着增加。在班级水平上，与健康对照组相比，ZQKX 组中的丹毒丝菌减少，而乳杆菌科的丰度增加。在家庭水平上，与健康受试者相比，XDYS 综合征患者的普氏菌属和柯林斯菌属减少，而链球菌显着增加。在 ZQKX 和 XDYS 综合征之间确定了五个不同的分类单元。我们认为肠道菌群有助于区分结直肠癌两种中医证型，可作为结直肠癌中医辨证治疗的生物学基础。
Colorectal cancer (CRC) is considered to be closely associated with alteration of intestinal microorganisms. The purpose of present study was to investigate the distribution of gut microbiota in the distinction of microbiota dysbiosis between two disease syndromes called Zheng-Qi-Kui-Xu(ZQKX) and Xie-Du-Yong-Sheng (XDYS). First, From February 2019 to June 2019, CRC patients presenting to the oncology department of Zhejiang Province Hospital of TCM who met the established inclusion and exclusion criteria were enrolled in this prospective study. After fresh stool specimens of healthy volunteers and CRC patients with ZQKX or XDYS syndorme were collected, 16S rRNA gene amplification and sequencing could be used to identify the diversity and abundance of gut microbiota among groups. The results demonstrated that the composition of the microbiota in general control group was superior to those in experimental groups. At the phylum level, a significantly increased abundance of Bacteroides was observed in healthy volunteers. At the class level, Erysipelothrix decreased while Lactobacillaceae showed increased abundance in the ZQKX group compared to healthy controls. At the family level, Prevotella Shan and Collins decreased while Streptococcus significantly increased in patients with XDYS syndrome compared to healthy subjects. Five differential taxa were identified between ZQKX and XDYS syndromes. We suggest that the gut microbiota contributes to the distinction between the two TCM syndromes of CRC, which can be used as a biological basis of TCM syndrome differentiation treatment in CRC.
登录
查看更多内容
微生物组和癌症。
DOI：
10.1038/nrc3610
发表时间：
2013-11
期刊：
Nature reviews. Cancer
影响因子：
--
作者：
通讯作者：
--
影响因子：
24.5
作者：
Flemer B;Lynch DB;Brown JM;Jeffery IB;Ryan FJ;Claesson MJ;O'Riordain M;Shanahan F;O'Toole PW
通讯作者：
O'Toole PW
影响因子：
4.2
作者：
Kasai, Chika;Sugimoto, Kazushi;Takase, Kojiro
通讯作者：
Takase, Kojiro
影响因子：
3.3
作者：
Han S;Gao J;Zhou Q;Liu S;Wen C;Yang X
通讯作者：
Yang X
综合微生物组和代谢组分析揭示了结直肠癌中共生细菌和代谢物之间的新相互作用
影响因子：
12.4
作者：
Yang, Yongzhi;Misra, Biswapriya B.;Ma, Yanlei
通讯作者：
Ma, Yanlei