qDTY₁.₁, a major QTL for rice grain yield under reproductive-stage drought stress with a consistent effect in multiple elite genetic backgrounds.
qDTY₁.₁, a major QTL for rice grain yield under reproductive-stage drought stress with a consistent effect in multiple elite genetic backgrounds.
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DOI：
10.1186/1471-2156-12-89
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发表时间：
2011-10-18
期刊：
影响因子：
2.9
通讯作者：
Kumar A
中科院分区：
文献类型：
--
作者：
Vikram P;Swamy BP;Dixit S;Ahmed HU;Teresa Sta Cruz M;Singh AK;Kumar A
干旱是造成水稻产量急剧下降的最重要的非生物胁迫之一。干旱条件下的粮食产量（戈伊）作为选择标准的适宜性在过去几年中已有报道。目前报道的水稻干旱戈伊QTL大多数是在低感品种的背景下进行的。这些QTL在多个高产干旱敏感品种中没有显示出类似的效应，因此限制了它们在标记辅助选择中的应用。以耐旱玉米品种N22与高产玉米品种Swarna、IR 64和MTU 1010杂交的3个F3：4作图群体为材料，在多个优良遗传背景下，研究了生殖期干旱胁迫对戈伊产量的遗传控制，旨在确定在多个优良遗传背景下表现一致效应的戈伊产量QTL。在2009年和2010年的干旱季节（DS），在IRRI的RS下对三个种群进行了表型分析。对于基因分型，使用SSR标记对N22/MTU 1010进行全基因组扫描，并对N22/Swarna和N22/IR 64进行混合分离分析。在3个群体中均检测到一个RS下的戈伊主效QTL qDTY1.1，位于水稻第1染色体上，位于RM 11943和RM 431两侧。在两年的组合分析中，在RS条件下，N22/Swarna、N22/IR 64和N22/MTU 1010的qDTY1.1平均产量的加性效应分别为29.3%、24.3%和16.1%。在N22/Swarna、N22/IR 64两年内以及DS 2009中的N22/MTU 1010中，qDTY1.1在非应激（NS）情况下也显示出对戈伊的积极影响。这是首次报道的水稻QTL，在RS和NS情况下，在多个精英遗传背景下的主要和一致的效果。QTL效应在不同遗传背景中的一致性使其成为用于标记辅助育种的合适候选者。
Drought is one of the most important abiotic stresses causing drastic reductions in yield in rainfed rice environments. The suitability of grain yield (GY) under drought as a selection criterion has been reported in the past few years. Most of the quantitative trait loci (QTLs) for GY under drought in rice reported so far has been in the background of low-yielding susceptible varieties. Such QTLs have not shown a similar effect in multiple high- yielding drought-susceptible varieties, thus limiting their use in marker-assisted selection. Genetic control of GY under reproductive-stage drought stress (RS) in elite genetic backgrounds was studied in three F3:4 mapping populations derived from crosses of N22, a drought-tolerant aus cultivar, with Swarna, IR64, and MTU1010, three high-yielding popular mega-varieties, with the aim to identify QTLs for GY under RS that show a consistent effect in multiple elite genetic backgrounds. Three populations were phenotyped under RS in the dry seasons (DS) of 2009 and 2010 at IRRI. For genotyping, whole-genome scans for N22/MTU1010 and bulked segregant analysis for N22/Swarna and N22/IR64 were employed using SSR markers. A major QTL for GY under RS, qDTY1.1, was identified on rice chromosome 1 flanked by RM11943 and RM431 in all three populations. In combined analysis over two years, qDTY1.1 showed an additive effect of 29.3%, 24.3%, and 16.1% of mean yield in N22/Swarna, N22/IR64, and N22/MTU1010, respectively, under RS. qDTY1.1 also showed a positive effect on GY in non-stress (NS) situations in N22/Swarna, N22/IR64 over both years, and N22/MTU1010 in DS2009. This is the first reported QTL in rice with a major and consistent effect in multiple elite genetic backgrounds under both RS and NS situations. Consistency of the QTL effect across different genetic backgrounds makes it a suitable candidate for use in marker-assisted breeding.
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