Comparative genome analysis reveals a conserved family of actin-like proteins in apicomplexan parasites.
Comparative genome analysis reveals a conserved family of actin-like proteins in apicomplexan parasites.
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比较基因组分析揭示了Apicomplexan寄生虫中保守的肌动蛋白样蛋白家族。
DOI：
10.1186/1471-2164-6-179
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发表时间：
2005-12-12
期刊：
影响因子：
4.4
通讯作者：
Sibley LD
中科院分区：
文献类型：
--
作者：
Gordon JL;Sibley LD
顶复合体门是一个早期分支的真核生物谱系，包含许多重要的人类和动物病原体。它们复杂的生命周期和独特的细胞骨架特征使它们区别于其他模式真核生物。顶复合体依靠基于动作蛋白的运动来进行细胞入侵，然而这个系统的调控在很大程度上仍然是未知的。因此，我们将工作重点集中在最近完成的弓形虫、疟原虫、隐孢子虫和氏杆菌基因组中肌动蛋白相关蛋白的鉴定上。对顶端复合体基因组的比较基因组学和系统发育研究表明，大多数只包含一个常规肌动蛋白，但它们每个都有8-10个额外的肌动蛋白相关蛋白。其中包括高度保守的Arp1蛋白（可能是保守的dynactin复合体的一部分）和Arp4和Arp6同源物（染色质重塑机制的亚基）。相反，顶复合体缺乏典型的Arp2或Arp3蛋白，这表明它们在细胞内寄生的进化过程中失去了Arp2/3肌动蛋白聚合复合体。这些肌动蛋白样蛋白（ALPs）中有7种对顶复合体来说是新的。它们与已知的Arp群没有系统发育上的联系，可能对这一重要的细胞内寄生虫群具有特定的功能。顶复合体中肌动蛋白样蛋白的巨大多样性表明肌动蛋白家族已经分化，在细胞内寄生虫的独特生物学中发挥着不同的作用。保守的Arps可能参与囊泡运输和基因表达，而顶复合体特异性的ALPs可能控制独特的生物学性状，如基于动作蛋白的滑翔运动。
The phylum Apicomplexa is an early-branching eukaryotic lineage that contains a number of important human and animal pathogens. Their complex life cycles and unique cytoskeletal features distinguish them from other model eukaryotes. Apicomplexans rely on actin-based motility for cell invasion, yet the regulation of this system remains largely unknown. Consequently, we focused our efforts on identifying actin-related proteins in the recently completed genomes of Toxoplasma gondii, Plasmodium spp., Cryptosporidium spp., and Theileria spp. Comparative genomic and phylogenetic studies of apicomplexan genomes reveals that most contain only a single conventional actin and yet they each have 8–10 additional actin-related proteins. Among these are a highly conserved Arp1 protein (likely part of a conserved dynactin complex), and Arp4 and Arp6 homologues (subunits of the chromatin-remodeling machinery). In contrast, apicomplexans lack canonical Arp2 or Arp3 proteins, suggesting they lost the Arp2/3 actin polymerization complex on their evolutionary path towards intracellular parasitism. Seven of these actin-like proteins (ALPs) are novel to apicomplexans. They show no phylogenetic associations to the known Arp groups and likely serve functions specific to this important group of intracellular parasites. The large diversity of actin-like proteins in apicomplexans suggests that the actin protein family has diverged to fulfill various roles in the unique biology of intracellular parasites. Conserved Arps likely participate in vesicular transport and gene expression, while apicomplexan-specific ALPs may control unique biological traits such as actin-based gliding motility.
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影响因子：
64.8
作者：
CLARK, SW;MEYER, DI
通讯作者：
MEYER, DI
真核生物寄生虫弓形虫的分泌流量：更少。
DOI：
10.1083/jcb.200112144
发表时间：
2002-05-13
期刊：
The Journal of cell biology
影响因子：
--
作者：
Joiner KA;Roos DS
通讯作者：
Roos DS
影响因子：
7.5
作者：
Frankel, S;Mooseker, MS
通讯作者：
Mooseker, MS
影响因子：
2.2
作者：
Kaplan, JE;Jones, JL;Dykewicz, CA
通讯作者：
Dykewicz, CA
影响因子：
7.8
作者：
Gill, S R;Schroer, T A;Szilak, I;Steuer, E R;Sheetz, M P;Cleveland, D W
通讯作者：
Cleveland, D W