Bacillus licheniformis FA6 Affects Zebrafish Lipid Metabolism through Promoting Acetyl-CoA Synthesis and Inhibiting β-Oxidation.
Bacillus licheniformis FA6 Affects Zebrafish Lipid Metabolism through Promoting Acetyl-CoA Synthesis and Inhibiting β-Oxidation.
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地衣芽孢杆菌 FA6 通过促进乙酰辅酶 A 合成和抑制 β 氧化影响斑马鱼脂质代谢
DOI：
10.3390/ijms24010673
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发表时间：
2022-12-30
影响因子：
5.6
通讯作者：
Wu, Shangong
中科院分区：
文献类型：
--
作者：
Chen, Sijia;Ye, Weidong;Clements, Kendall D.;Zan, Ziye;Zhao, Weishan;Zou, Hong;Wang, Guitang;Wu, Shangong
肠道微生物群有助于能量代谢，但所涉及的分子机制仍不清楚。芽孢杆菌属的细菌调节宿主中的脂质代谢，因此通常用作有益的益生菌补充剂。本研究以地衣芽孢杆菌FA 6为研究对象，探讨其对斑马鱼脂质代谢的调节作用。结合16 SrRNA高通量测序、micro-CT扫描、代谢参数测定和基因表达分析，我们证明了B.地衣菌FA 6改变了斑马鱼的肠道微生物群组成，并增加了厚壁菌门/拟杆菌门的比例和脂质积累。在代谢物方面，B.地衣状菌FA 6似乎促进乙酸盐的产生，这增加了乙酰辅酶A水平并促进肝脏中的脂质合成。相反，添加B.地衣降低了肉毒碱的水平，这反过来又减少了肝脏中的脂肪酸氧化。在分子水平上，B。地衣芽孢杆菌FA 6上调调节脂肪酸从头合成的关键基因，下调编码脂肪酸β-氧化的关键限速酶的基因，从而促进脂质合成并减少脂肪酸氧化。总体上，我们的结果表明，B。Licheniformis FA 6通过促进脂质合成和减少β-氧化促进斑马鱼脂质积累。
The intestinal microbiota contributes to energy metabolism, but the molecular mechanisms involved remain less clear. Bacteria of the genus Bacillus regulate lipid metabolism in the host and are thus commonly used as beneficial probiotic supplements. In the present study, Bacillus licheniformis FA6 was selected to assess its role in modulating lipid metabolism of zebrafish (Danio rerio). Combining 16S rRNA high-throughput sequencing, micro-CT scan, metabolic parameters measurement, and gene expression analysis, we demonstrated that B. licheniformis FA6 changed the gut microbiota composition of zebrafish and increased both the Firmicutes/Bacteroidetes ratio and lipid accumulation. In terms of metabolites, B. licheniformis FA6 appeared to promote acetate production, which increased acetyl-CoA levels and promoted lipid synthesis in the liver. In contrast, addition of B. licheniformis lowered carnitine levels, which in turn reduced fatty acid oxidation in the liver. At a molecular level, B. licheniformis FA6 upregulated key genes regulating de novo fatty acid synthesis and downregulated genes encoding key rate-limiting enzymes of fatty acid β-oxidation, thereby promoting lipid synthesis and reducing fatty acid oxidation. Generally, our results reveal that B. licheniformis FA6 promotes lipid accumulation in zebrafish through improving lipid synthesis and reducing β-oxidation.
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DOI：
10.1093/bioinformatics/btu638
发表时间：
2015-01-15
期刊：
Bioinformatics (Oxford, England)
影响因子：
--
作者：
Anders S;Pyl PT;Huber W
通讯作者：
Huber W
影响因子：
16.6
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DOI：
10.1038/nrmicro2746
发表时间：
2012-04-11
期刊：
Nature reviews. Microbiology
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10.1007/bf00302552
发表时间：
1994-10-01
期刊：
JOURNAL OF COMPARATIVE PHYSIOLOGY B-BIOCHEMICAL SYSTEMIC AND ENVIRONMENTAL PHYSIOLOGY
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作者：
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DOI：
10.1099/00221287-144-1-83
发表时间：
1998-01-01
期刊：
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