Making Thymus Visible: Understanding T-Cell Development from a New Perspective.
Making Thymus Visible: Understanding T-Cell Development from a New Perspective.
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DOI：
10.3389/fimmu.2018.00375
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发表时间：
2018
影响因子：
7.3
通讯作者：
Bajoghli B
中科院分区：
文献类型：
--
作者：
Aghaallaei N;Bajoghli B
T细胞的发育伴随着高度有序的迁移模式。类胸腺祖细胞必须遵循一个精确的旅程;从造血组织开始，它们向胸腺移动，然后迁移到不同的胸腺微环境中，在那里它们接收分化为幼稚T细胞所需的信号和线索。了解这些细胞在何处、何时以及如何做出方向性“决定”是理解T细胞发育的关键。这样的见解可以通过在各种条件下直接观察其环境中的发育中的T细胞并遵循特定的实验操作来获得。在过去的十年中，已经开发了几个模型系统，以解决使用成像方法的T细胞发育的时间和空间方面。在这篇文章中，我们讨论了这些系统的优点和局限性，并强调了最近建立的一个特别强大的体内模型。该模型系统能够以非侵入性和定量的方式同时研究所有胸腺细胞的迁移行为，从而可以进行系统水平的研究，揭示发育和疾病期间T细胞动力学的基本原理。
T-cell development is coupled with a highly ordered migratory pattern. Lymphoid progenitors must follow a precise journey; starting from the hematopoietic tissue, they move toward the thymus and then migrate into and out of distinct thymic microenvironments, where they receive signals and cues required for their differentiation into naïve T-cells. Knowing where, when, and how these cells make directional “decisions” is key to understanding T-cell development. Such insights can be gained by directly observing developing T-cells within their environment under various conditions and following specific experimental manipulations. In the last decade, several model systems have been developed to address temporal and spatial aspects of T-cell development using imaging approaches. In this perspective article, we discuss the advantages and limitations of these systems and highlight a particularly powerful in vivo model that has been recently established. This model system enables the migratory behavior of all thymocytes to be studied simultaneously in a noninvasive and quantitative manner, making it possible to perform systems-level studies that reveal fundamental principles governing T-cell dynamics during development and in disease.
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