Heterotrimeric G-Protein γ Subunit CsGG3.2 Positively Regulates the Expression of CBF Genes and Chilling Tolerance in Cucumber.
Heterotrimeric G-Protein γ Subunit CsGG3.2 Positively Regulates the Expression of CBF Genes and Chilling Tolerance in Cucumber.
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DOI：
10.3389/fpls.2018.00488
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发表时间：
2018
影响因子：
5.6
通讯作者：
Yu X
中科院分区：
文献类型：
--
作者：
Bai L;Liu Y;Mu Y;Anwar A;He C;Yan Y;Li Y;Yu X
异源三聚体鸟嘌呤核苷酸结合蛋白（G蛋白）由α（Gα）、β（Gβ）和γ（Gγ）亚基组成，是真核生物中介导细胞对多种刺激（如寒冷）应答的中心信号转导子。植物Gγ亚基分为A、B和C三种结构不同的类型，为异源三聚体复合物提供适当的细胞定位和功能特异性。在此，我们证明了C型Gγ亚基CsGG 3.2参与了CBF调节子的调节和黄瓜（Cucumis sativus L.）对冷胁迫的耐受性。我们发现，CsGG3.2转录丰度正诱导冷处理。组成型过表达CsGG3.2的转基因黄瓜植株（T1）表现出对低温条件的耐受性和CBF基因及其调节子的表达增加。抗氧化酶，即超氧化物歧化酶、过氧化氢酶、过氧化物酶和谷胱甘肽还原酶活性在低温胁迫下增加。转基因植株体内活性氧、氧自由基和H2O2的产生以及膜脂过氧化（MDA）的产生均减少，表明转基因植株体内有较好的抗氧化系统来科普低温胁迫引起的氧化损伤。这些发现为CsGG3.2在介导植物细胞冷信号转导中的关键作用提供了证据。
Heterotrimeric guanine nucleotide-binding proteins (G proteins) composed of alpha (Gα), beta (Gβ), and gamma (Gγ) subunits are central signal transducers mediating the cellular response to multiple stimuli, such as cold, in eukaryotes. Plant Gγ subunits, divided into A, B, and C three structurally distinct types, provide proper cellular localization and functional specificity to the heterotrimer complex. Here, we demonstrate that a type C Gγ subunit CsGG3.2 is involved in the regulation of the CBF regulon and plant tolerance to cold stresses in cucumber (Cucumis sativus L.). We showed that CsGG3.2 transcript abundance was positively induced by cold treatments. Transgenic cucumber plants (T1) constitutively over-expressing CsGG3.2 exhibits tolerance to chilling conditions and increased expression of CBF genes and their regulon. Antioxidative enzymes, i.e., superoxide dismutase, catalase, peroxidase, and glutathione reductase activities increased in cold-stressed transgenic plants. The reactive oxygen species, oxygen free radical and H2O2, production, as well as membrane lipid peroxidation (MDA) production decreased in transgenic plants, suggesting a better antioxidant system to cope the oxidative-damages caused by cold stress. These findings provide evidence for a critical role of CsGG3.2 in mediating cold signal transduction in plant cells.
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影响因子：
5.6
作者：
Mu Y;Liu Y;Bai L;Li S;He C;Yan Y;Yu X;Li Y
通讯作者：
Li Y
影响因子：
64.5
作者：
Ma, Yun;Dai, Xiaoyan;Chong, Kang
通讯作者：
Chong, Kang
影响因子：
5.2
作者：
Baek, KH;Skinner, DZ
通讯作者：
Skinner, DZ
影响因子：
--
作者：
Kang Guozhang;Zhu Zhonghou;Ren Jiangping
通讯作者：
Ren Jiangping
影响因子：
3.9
作者：
Soltesz, A.;Timar, I.;Galiba, G.
通讯作者：
Galiba, G.