Dissecting the mechanisms responsible for the multiple insecticide resistance phenotype in Anopheles gambiae s.s., M form, from Vallée du Kou, Burkina Faso.
Dissecting the mechanisms responsible for the multiple insecticide resistance phenotype in Anopheles gambiae s.s., M form, from Vallée du Kou, Burkina Faso.
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DOI：
10.1016/j.gene.2013.01.036
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发表时间：
2013-04-25
期刊：
影响因子：
3.5
通讯作者：
Wondji CS
中科院分区：
文献类型：
--
作者：
Kwiatkowska RM;Platt N;Poupardin R;Irving H;Dabire RK;Mitchell S;Jones CM;Diabaté A;Ranson H;Wondji CS
除靶点突变外，疟疾主要媒介冈比亚按蚊的杀虫剂抗药性机制在布基纳法索基本上仍不明确。在这里，我们检测到在Vallée du Kou（VK）的高流行率的抗药性拟除虫菊酯，滴滴涕和狄氏剂，中等水平的氨基甲酸酯和完全敏感的有机磷。观察到高频率的L1014 F kdr（75%）和Rdl（87%）突变，显示与拟除虫菊酯/DDT和狄氏剂抗性强相关。即使在氨基甲酸酯抗性蚊子中，ace 1 R突变的频率也很低。微阵列分析鉴定了VK中显著过度转录的基因。这些包括细胞色素P450基因，CYP 6P 3和CYP 6 Z2，以前与拟除虫菊酯抗性相关。基因本体（GO）富集分析表明，神经递质活性升高与抗性相关，与靶位点抗性基因如乙酰胆碱酯酶和GABA受体的过度转录有关。以前与拟除虫菊酯抗性相关的淡色库蚊视紫红质受体基因也在VK中过度转录。这项研究强调了疟疾病媒产生多重抗药性的复杂机制网络，在设计和实施抗药性控制战略时应考虑到这些信息。VK对拟除虫菊酯、DDT和狄氏剂的耐药性高，氨基甲酸酯的耐药性中等VK中kdr和Rdl突变频率高，与耐药性强相关VK中上调基因的微阵列检测，特别是CYP 6P 3和CYP 6 Z2 P450 s VK中神经递质活性GO术语富集; Ace-1/GABA受体上调VK中与淡色库蚊耐药性相关的视紫红质基因
With the exception of target site mutations, insecticide resistance mechanisms in the principle malaria vector Anopheles gambiae, remains largely uncharacterized in Burkina Faso. Here we detected high prevalence of resistance in Vallée du Kou (VK) to pyrethroids, DDT and dieldrin, moderate level for carbamates and full susceptibility to organophosphates. High frequencies of L1014F kdr (75%) and Rdl (87%) mutations were observed showing strong correlation with pyrethroids/DDT and dieldrin resistance. The frequency of ace1R mutation was low even in carbamate resistant mosquitoes. Microarray analysis identified genes significantly over-transcribed in VK. These include the cytochrome P450 genes, CYP6P3 and CYP6Z2, previously associated with pyrethroid resistance. Gene Ontology (GO) enrichment analysis suggested that elevated neurotransmitter activity is associated with resistance, with the over-transcription of target site resistance genes such as acetylcholinesterase and the GABA receptor. A rhodopsin receptor gene previously associated with pyrethroid resistance in Culex pipiens pallens was also over-transcribed in VK. This study highlights the complex network of mechanisms conferring multiple resistance in malaria vectors and such information should be taken into account when designing and implementing resistance control strategies. ► High pyrethroids, DDT and dieldrin resistance in VK, moderate for carbamate ► High frequencies kdr and Rdl mutations in VK with strong correlation with resistance ► Detection using microarray of genes upregulated in VK notably CYP6P3 and CYP6Z2 P450s ► Neurotransmitter activity GO terms enriched in VK; Ace-1/GABA receptor upregulated ► A rhodopsin gene associated with resistance in Culex p. pallens upregulated in VK
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影响因子：
3.7
作者：
Giraudo M;Califano J;Hilliou F;Tran T;Taquet N;Feyereisen R;Le Goff G
通讯作者：
Le Goff G
影响因子：
2.6
作者：
Du, W;Awolola, TS;Zheng, L
通讯作者：
Zheng, L
影响因子：
4.7
作者：
Hu, Xiaobang;Sun, Yan;Zhu, Changliang
通讯作者：
Zhu, Changliang
现场捕获的耐氯菊酯抗蚊子冈比亚过表达CYP6P3，一种代谢拟除虫菊酯的P450。
影响因子：
4.5
作者：
Mueller, Pie;Warr, Emma;Stevenson, Bradley J.;Pignatelli, Patricia M.;Morgan, John C.;Steven, Andrew;Yawson, Alexander E.;Mitchell, Sara N.;Ranson, Hilary;Hemingway, Janet;Paine, Mark J. I.;Donnelly, Martin J.
通讯作者：
Donnelly, Martin J.
影响因子：
14.8
作者：
Huang, Da Wei;Sherman, Brad T.;Lempicki, Richard A.
通讯作者：
Lempicki, Richard A.