HypoMap-a unified single-cell gene expression atlas of the murine hypothalamus.
HypoMap-a unified single-cell gene expression atlas of the murine hypothalamus.
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DOI：
10.1038/s42255-022-00657-y
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发表时间：
2022-10
影响因子：
20.8
通讯作者：
Bruening, Jens C.
中科院分区：
文献类型：
--
作者：
Steuernagel, Lukas;Lam, Brian Y. H.;Klemm, Paul;Dowsett, Georgina K. C.;Bauder, Corinna A.;Tadross, John A.;Hitschfeld, Tamara Sotelo;Martin, Almudena del Rio;Chen, Weiyi;de Solis, Alain J.;Fenselau, Henning;Davidsen, Peter;Cimino, Irene;Kohnke, Sara N.;Rimmington, Debra;Coll, Anthony P.;Beyer, Andreas;Yeo, Giles S. H.;Bruening, Jens C.
下丘脑在协调基本身体功能方面起着关键作用。尽管单细胞技术最近取得了进展，但对这一大脑区域的异质细胞类型，特别是神经元亚型，仍然缺乏统一的目录和分子表征。在这里，我们提出了一个完整的参考图谱，“HypoMap”，小鼠下丘脑，由384,925个细胞组成，具有纳入新的附加实验的能力。我们通过比较Smart-Seq+Fluidigm C1收集的数据和选择的具有不同程度细胞异质性的神经细胞类型的大量RNA测序来验证HypoMap。最后，通过HypoMap，我们确定了表达胰高血糖素样肽-1受体（Glp1r）和前孕激素受体（Pnoc）的神经元类别，并使用单分子原位杂交验证它们。总之，HypoMap为小鼠下丘脑细胞类型的系统功能注释提供了一个统一的框架，它可以作为揭示下丘脑神经回路的功能组织和识别治疗代谢紊乱的药物靶点的重要平台。Steuernagel和Lam等人提出了HypoMap，这是一个基于384,925个下丘脑细胞的综合参考图谱，这些细胞来自公开的单细胞测序数据集。
The hypothalamus plays a key role in coordinating fundamental body functions. Despite recent progress in single-cell technologies, a unified catalog and molecular characterization of the heterogeneous cell types and, specifically, neuronal subtypes in this brain region are still lacking. Here, we present an integrated reference atlas, ‘HypoMap,’ of the murine hypothalamus, consisting of 384,925 cells, with the ability to incorporate new additional experiments. We validate HypoMap by comparing data collected from Smart-Seq+Fluidigm C1 and bulk RNA sequencing of selected neuronal cell types with different degrees of cellular heterogeneity. Finally, via HypoMap, we identify classes of neurons expressing glucagon-like peptide-1 receptor (Glp1r) and prepronociceptin (Pnoc), and validate them using single-molecule in situ hybridization. Collectively, HypoMap provides a unified framework for the systematic functional annotation of murine hypothalamic cell types, and it can serve as an important platform to unravel the functional organization of hypothalamic neurocircuits and to identify druggable targets for treating metabolic disorders. Steuernagel and Lam et al. present HypoMap, an integrated reference atlas of the murine hypothalamus based on 384,925 hypothalamic cells from publicly available single-cell sequencing datasets.
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影响因子：
8.8
作者：
Kohnke S;Buller S;Nuzzaci D;Ridley K;Lam B;Pivonkova H;Bentsen MA;Alonge KM;Zhao C;Tadross J;Holmqvist S;Shimizu T;Hathaway H;Li H;Macklin W;Schwartz MW;Richardson WD;Yeo GSH;Franklin RJM;Karadottir RT;Rowitch DH;Blouet C
通讯作者：
Blouet C
下丘脑胰高血糖素样肽1受体足够，但对于调节小鼠的能量平衡和葡萄糖稳态不需要。
影响因子：
7.7
作者：
Burmeister MA;Ayala JE;Smouse H;Landivar-Rocha A;Brown JD;Drucker DJ;Stoffers DA;Sandoval DA;Seeley RJ;Ayala JE
通讯作者：
Ayala JE
影响因子：
20.8
作者：
Lee, Stephen D.;Priest, Christina;Hong, Cynthia
通讯作者：
Hong, Cynthia
影响因子：
7.7
作者：
Affinati AH;Sabatini PV;True C;Tomlinson AJ;Kirigiti M;Lindsley SR;Li C;Olson DP;Kievit P;Myers MG;Rupp AC
通讯作者：
Rupp AC
影响因子：
48
作者：
Korsunsky, Ilya;Millard, Nghia;Raychaudhuri, Soumya
通讯作者：
Raychaudhuri, Soumya