Use of autologous mesenchymal stem cells derived from bone marrow for the treatment of naturally injured spinal cord in dogs.
Use of autologous mesenchymal stem cells derived from bone marrow for the treatment of naturally injured spinal cord in dogs.
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DOI：
10.1155/2014/437521
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发表时间：
2014
影响因子：
4.3
通讯作者：
Soares MB
中科院分区：
文献类型：
--
作者：
Penha EM;Meira CS;Guimarães ET;Mendonça MV;Gravely FA;Pinheiro CM;Pinheiro TM;Barrouin-Melo SM;Ribeiro-Dos-Santos R;Soares MB
干细胞在损伤修复中的应用已经得到了广泛的研究。在这里，我们研究了自体骨髓间充质干细胞(MSC)移植对4只犬的自然创伤性脊髓损伤的治疗效果。体外培养间充质干细胞，检测细胞增殖率和细胞存活率。制备细胞悬浮液，并通过手术将其注入脊髓。对动物进行临床评估和核磁共振检查。手术和骨髓间充质干细胞移植10天后，我们观察到脉络膜反射逐渐恢复，浅层和深层疼痛反应减弱，尽管在共济失调的后肢运动反应中仍有低本体感觉反射和高反射。随着时间的推移，每只狗都表现出了这些收益的改善。意识反射恢复的同时，四只狗中有两只的肠道和膀胱功能有了适度的改善。经过第18个月的临床监测，我们观察到四只狗中有三只的临床症状有显著改善，并伴有运动能力的改善。然而，磁共振成像的改变与临床进展无关。我们的结果表明，MSC是脊髓损伤后干细胞治疗的潜在候选者。
The use of stem cells in injury repair has been extensively investigated. Here, we examined the therapeutic effects of autologous bone marrow mesenchymal stem cells (MSC) transplantation in four dogs with natural traumatic spinal cord injuries. MSC were cultured in vitro, and proliferation rate and cell viability were evaluated. Cell suspensions were prepared and surgically administered into the spinal cord. The animals were clinically evaluated and examined by nuclear magnetic resonance. Ten days after the surgical procedure and MSC transplantation, we observed a progressive recovery of the panniculus reflex and diminished superficial and deep pain response, although there were still low proprioceptive reflexes in addition to a hyperreflex in the ataxic hind limb movement responses. Each dog demonstrated an improvement in these gains over time. Conscious reflex recovery occurred simultaneously with moderate improvement in intestine and urinary bladder functions in two of the four dogs. By the 18th month of clinical monitoring, we observed a remarkable clinical amelioration accompanied by improved movement, in three of the four dogs. However, no clinical gain was associated with alterations in magnetic resonance imaging. Our results indicate that MSC are potential candidates for the stem cell therapy following spinal cord injury.
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影响因子：
1.8
作者：
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通讯作者：
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影响因子：
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