Presence of B. thailandensis and B. thailandensis expressing B. pseudomallei-like capsular polysaccharide in Thailand, and their associations with serological response to B. pseudomallei.
Presence of B. thailandensis and B. thailandensis expressing B. pseudomallei-like capsular polysaccharide in Thailand, and their associations with serological response to B. pseudomallei.
复制标题
DOI：
10.1371/journal.pntd.0006193
复制
发表时间：
2018-01
影响因子：
3.8
通讯作者：
Limmathurotsakul D
中科院分区：
文献类型：
--
作者：
Hantrakun V;Thaipadungpanit J;Rongkard P;Srilohasin P;Amornchai P;Langla S;Mukaka M;Chantratita N;Wuthiekanun V;Dance DAB;Day NPJ;Peacock SJ;Limmathurotsakul D
鼻疽伯克霍尔德氏菌是一种环境革兰氏阴性杆菌，也是类鼻疽的病因。泰国芽孢杆菌（B. thailandensis）的一些菌株表达类鼻疽芽孢杆菌样荚膜多糖（BTCV），也常见于东南亚环境中，但被认为是非致病性的。该研究的目的是确定泰国伯克利氏菌及其荚膜变种在泰国的分布，并调查其存在是否与类鼻疽伯克氏菌的血清学反应有关。我们评估了泰国东北部 (n = 21)、东部 (n = 19) 和中部 (n = 21) 61 个稻田中的 B.pseudomallei 和 B.thailandensis 的存在情况。我们在泰国东部和中部的稻田中发现了 BTCV，但在泰国东北部没有发现。 14 个视野仅对类鼻疽伯克氏菌培养呈阳性，8 个对鼻疽伯克氏菌单独培养呈阳性，11 个对拟鼻疽伯克氏菌和泰国鼻疽伯克氏菌两者均呈阳性，6 个对鼻疽伯克氏菌和 BTCV 均呈培养阳性，以及 5 个对拟鼻疽伯克氏菌、泰国鼻疽伯克氏菌和 BTCV 均呈培养阳性。使用间接血凝试验 (IHA) 对在研究田地工作的 96 名农民进行血清学检测，结果表明，在类鼻疽伯克氏菌阳性田地工作的农民比在类鼻疽伯克氏菌阴性田地工作的农民具有更高的 IHA 滴度（中位数 1:40 [范围：<1:10–1:640] 与 <1:10 [范围：<1:10–1:320]，p = 0.002）。在多变量有序 Logistic 回归模型中，IHA 滴度与类鼻疽杆菌的存在显着相关（aOR = 3.7；95% CI 1.8–7.8，p = 0.001），但与泰国鼻疽杆菌（p = 0.32）或 BTCV（p = 0.32）的存在无关。为测试的 27 个 BTCV 分离株确定了一种序列类型 (696)。这是BTCV在泰国的第一份报告。在同一田地中同时存在类鼻疽杆菌和泰国鼻疽杆菌并不罕见。我们的研究结果表明，泰国健康稻农的 IHA 阳性与稻田中的类鼻疽杆菌有关，而不是与泰国鼻疽杆菌或 BTCV 相关。泰国伯克霍尔德杆菌是一种非致病性土壤细菌，与鼻疽伯克霍尔德杆菌（类鼻疽的病因）密切相关。在小鼠模型中，接种表达类鼻疽芽孢杆菌样荚膜多糖 (BTCV) 的泰国芽孢杆菌变种可诱导抗体并部分预防类鼻疽。在这里，我们评估了泰国东北部、东部和中部 61 个稻田中的拟鼻疽伯克虫、泰国伯克利氏杆菌和 BTCV 的存在情况，确定它们是否共存，以及它们的存在是否与农民的血清学反应有关。我们首次报告泰国存在 BTCV，并描述了这三种生物体的分布。这些生物体在同一稻田中共存的情况并不少见。我们的研究结果表明，泰国健康稻农的间接血凝试验（一种常用于检测类鼻疽伯克氏菌抗体的测试）的血清学阳性与接触类鼻疽伯克氏菌有关，但与接触泰国鼻疽伯克氏菌或 BTCV 无关。
Burkholderia pseudomallei is an environmental Gram-negative bacillus and the cause of melioidosis. B. thailandensis, some strains of which express a B. pseudomallei-like capsular polysaccharide (BTCV), is also commonly found in the environment in Southeast Asia but is considered non-pathogenic. The aim of the study was to determine the distribution of B. thailandensis and its capsular variant in Thailand and investigate whether its presence is associated with a serological response to B. pseudomallei. We evaluated the presence of B. pseudomallei and B. thailandensis in 61 rice fields in Northeast (n = 21), East (n = 19) and Central (n = 21) Thailand. We found BTCV in rice fields in East and Central but not Northeast Thailand. Fourteen fields were culture positive for B. pseudomallei alone, 8 for B. thailandensis alone, 11 for both B. pseudomallei and B. thailandensis, 6 for both B. thailandensis and BTCV, and 5 for B. pseudomallei, B. thailandensis and BTCV. Serological testing using the indirect hemagglutination assay (IHA) of 96 farmers who worked in the study fields demonstrated that farmers who worked in B. pseudomallei-positive fields had higher IHA titers than those who worked in B. pseudomallei-negative fields (median 1:40 [range: <1:10–1:640] vs. <1:10 [range: <1:10–1:320], p = 0.002). In a multivariable ordered logistic regression model, IHA titers were significantly associated with the presence of B. pseudomallei (aOR = 3.7; 95% CI 1.8–7.8, p = 0.001) but were not associated with presence of B. thailandensis (p = 0.32) or BTCV (p = 0.32). One sequence type (696) was identified for the 27 BTCV isolates tested. This is the first report of BTCV in Thailand. The presence of B. pseudomallei and B. thailandensis in the same field was not uncommon. Our findings suggest that IHA positivity of healthy rice farmers in Thailand is associated with the presence of B. pseudomallei in rice fields rather than B. thailandensis or BTCV. Burkholderia thailandensis is a non-pathogenic soil-dwelling bacterium and is genetically closely related to Burkholderia pseudomallei, the cause of melioidosis. In mouse models, inoculation of a variant of B. thailandensis which express a B. pseudomallei-like capsular polysaccharide (BTCV) induces antibodies and partial protection against melioidosis. Here, we evaluated the presence of B. pseudomallei, B. thailandensis, and BTCV in 61 rice fields in Northeast, East and Central Thailand, determined whether they co-existed, and if their presence was associated with a serological response in farmers. We report the presence of BTCV in Thailand for the first time and describe the distribution of the three organisms. Co-localization between these organisms in the same rice fields was not uncommon. Our findings suggested that serological positivity based on the indirect hemagglutination assay (a test commonly used to detect antibodies to B. pseudomallei) in healthy rice farmers in Thailand was associated with exposure to B. pseudomallei, but not exposure to B. thailandensis or BTCV.
登录
查看更多内容
影响因子：
--
作者：
Bursac, Zoran;Gauss, C Heath;Williams, David Keith;Hosmer, David W
通讯作者：
Hosmer, David W
影响因子：
4.4
作者：
Hantrakun V;Rongkard P;Oyuchua M;Amornchai P;Lim C;Wuthiekanun V;Day NP;Peacock SJ;Limmathurotsakul D
通讯作者：
Limmathurotsakul D
影响因子：
3.1
作者：
Reckseidler, SL;DeShazer, D;Woods, DE
通讯作者：
Woods, DE
影响因子：
--
作者：
Scott, Andrew E.;Laws, Thomas R.;Atkins, Timothy P.
通讯作者：
Atkins, Timothy P.
影响因子：
3
作者：
Anuntagool, N;Naigowit, P;Sirisinha, S
通讯作者：
Sirisinha, S