Meta-analysis of grain yield QTL identified during agricultural drought in grasses showed consensus.
Meta-analysis of grain yield QTL identified during agricultural drought in grasses showed consensus.
复制标题
DOI：
10.1186/1471-2164-12-319
复制
发表时间：
2011-06-16
期刊：
影响因子：
4.4
通讯作者：
Kumar A
中科院分区：
文献类型：
--
作者：
Swamy BP;Vikram P;Dixit S;Ahmed HU;Kumar A
近年来，人们致力于鉴定水稻干旱条件下产量的大效应QTL。然而，跨环境和遗传背景鉴定最精确和一致的 QTL 对于其在标记辅助选择中的成功应用至关重要。在本研究中，尝试通过应用全基因组QTL荟萃分析方法来定位与干旱条件下产量增加相关的一致QTL区域。 15 个图谱的整合产生了具有 531 个标记的共有图谱，图谱总长度为 1821 cM。将 15 项研究中报告的 53 个产量 QTL 投影到共识图上并进行荟萃分析。在7条染色体上获得了14个元QTL。 MQTL1.2、MQTL1.3、MQTL1.4 和 MQTL12.1 约为 700 kb，对应于 1.8 至 5 cM 的相当小的遗传距离，它们适合用于标记辅助选择 (MAS)。干旱条件下谷物产量的元 QTL 与早期报道中干旱条件下根和叶形态性状鉴定的至少一个元 QTL 一致。对一组随机耐旱品系的主效应 QTL 的验证表明，每个品系中至少存在一个主要 QTL。 DTY12.1 存在于 85% 的品系中，其次是 DTY4.1 存在于 79% 的品系中，DTY1.1 存在于 64% 的品系中。与其他谷物的meta-QTL基因组比较表明，MQTL1.4和MQTL3.2的同源区域分别具有玉米、小麦和大麦干旱条件下谷物产量的QTL。通过比较基因组学方法分析meta-QTL区域的基因，并推导出干旱条件下谷物产量的候选基因。在大多数元QTL中发现了三组基因，即胁迫诱导基因、生长发育相关基因和糖转运相关基因。具有较小遗传和物理间隔的元 QTL 可用于单独和组合的标记辅助选择。主效应 QTL 的验证和比较基因组学证实了它们在物种内和物种间的一致性。入围的候选基因可以被克隆，以揭示干旱条件下调节粮食产量的分子机制。
In the last few years, efforts have been made to identify large effect QTL for grain yield under drought in rice. However, identification of most precise and consistent QTL across the environments and genetics backgrounds is essential for their successful use in Marker-assisted Selection. In this study, an attempt was made to locate consistent QTL regions associated with yield increase under drought by applying a genome-wide QTL meta-analysis approach. The integration of 15 maps resulted in a consensus map with 531 markers and a total map length of 1821 cM. Fifty-three yield QTL reported in 15 studies were projected on a consensus map and meta-analysis was performed. Fourteen meta-QTL were obtained on seven chromosomes. MQTL1.2, MQTL1.3, MQTL1.4, and MQTL12.1 were around 700 kb and corresponded to a reasonably small genetic distance of 1.8 to 5 cM and they are suitable for use in marker-assisted selection (MAS). The meta-QTL for grain yield under drought coincided with at least one of the meta-QTL identified for root and leaf morphology traits under drought in earlier reports. Validation of major-effect QTL on a panel of random drought-tolerant lines revealed the presence of at least one major QTL in each line. DTY12.1 was present in 85% of the lines, followed by DTY4.1 in 79% and DTY1.1 in 64% of the lines. Comparative genomics of meta-QTL with other cereals revealed that the homologous regions of MQTL1.4 and MQTL3.2 had QTL for grain yield under drought in maize, wheat, and barley respectively. The genes in the meta-QTL regions were analyzed by a comparative genomics approach and candidate genes were deduced for grain yield under drought. Three groups of genes such as stress-inducible genes, growth and development-related genes, and sugar transport-related genes were found in clusters in most of the meta-QTL. Meta-QTL with small genetic and physical intervals could be useful in Marker-assisted selection individually and in combinations. Validation and comparative genomics of the major-effect QTL confirmed their consistency within and across the species. The shortlisted candidate genes can be cloned to unravel the molecular mechanism regulating grain yield under drought.
登录
查看更多内容
影响因子：
7.4
作者：
Jain, Mukesh;Nijhawan, Aashima;Khurana, Jitendra P.
通讯作者：
Khurana, Jitendra P.
影响因子：
3.1
作者：
Loeffler, Martin;Schoen, Chris-Carolin;Miedaner, Thomas
通讯作者：
Miedaner, Thomas
通过荟萃分析，在单个映射水稻中，在干旱相关QTL位置的分辨率提高了。
影响因子：
4.4
作者：
Khowaja FS;Norton GJ;Courtois B;Price AH
通讯作者：
Price AH
影响因子：
6.8
作者：
AKAIKE, H
通讯作者：
AKAIKE, H
影响因子：
2.3
作者：
Podlich, DW;Winkler, CR;Cooper, M
通讯作者：
Cooper, M