Genetic variability in the sdrD gene in Staphylococcus aureus from healthy nasal carriers.
Genetic variability in the sdrD gene in Staphylococcus aureus from healthy nasal carriers.
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DOI：
10.1186/s12866-018-1179-7
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发表时间：
2018-04-16
期刊：
影响因子：
4.2
通讯作者：
Hanssen AM
中科院分区：
文献类型：
--
作者：
Ajayi C;Åberg E;Askarian F;Sollid JUE;Johannessen M;Hanssen AM
金黄色葡萄球菌细胞壁锚定丝氨酸天冬氨酸重复序列蛋白D （SdrD）是识别黏附基质分子的微生物表面组分之一。它与细菌的粘附和毒力有关。然而，金黄色葡萄球菌sdrD基因在健康携带者分离株中的遗传变异程度尚不清楚。本研究的目的是评估来自健康鼻带菌者的金黄色葡萄球菌中sdrD基因的等位基因变异。对48株健康携带金黄色葡萄球菌分离株的sdra区进行了分析，并将其分为7个变异。sdrD - A区的变化主要集中在N2和N3亚域。对代表性菌株的全基因序列分析显示，SD重复序列和B重复序列的EF基序存在差异。计算机结构建模表明，7个变体的SdrD结构没有差异。在三维结构上的变化氨基酸残基映射表明，这些变化位于表面，存在于N2-N3子结构域之间的凹槽内，主要分布在N3子结构域上。在体外细胞粘附实验中，使用表达各种sdrD基因变体的NCTC 8325-4∆sdrD菌株对角质形成细胞的粘附进行比较，结果表明只有两种补体在粘附方面存在显著差异，而其他补体在粘附方面没有显著差异。这项研究提供了从健康的鼻腔携带者分离的金黄色葡萄球菌不同区域的序列变化的证据。正确理解这些变异是研究金黄色葡萄球菌发病机制的必要条件。本文的在线版本（10.1186/s12866-018-1179-7）包含补充材料，授权用户可以使用。
Staphylococcus aureus cell wall anchored Serine Aspartate repeat containing protein D (SdrD) is a member of the microbial surface component recognising adhesive matrix molecules (MSCRAMMs). It is involved in the bacterial adhesion and virulence. However the extent of genetic variation in S. aureus sdrD gene within isolates from healthy carriers are not known. The aim of this study was to evaluate allelic variation of the sdrD gene among S. aureus from healthy nasal carriers. The sdrD A region from 48 S. aureus isolates from healthy carriers were analysed and classified into seven variants. Variations in the sdrD A region were concentrated in the N2 and N3 subdomains. Sequence analysis of the entire sdrD gene of representative isolates revealed variations in the SD repeat and the EF motifs of the B repeat. In silico structural modelling indicates that there are no differences in the SdrD structure of the 7 variants. Variable amino acid residues mapped onto the 3D structure revealed that the variations are surface located, exist within the groove between the N2-N3 subdomains and distributed mainly on the N3 subdomain. Comparison of adhesion to keratinocytes in an in vitro cell adhesion assay, using NCTC 8325–4∆sdrD strains expressing the various sdrD gene variants, indicated a significant difference between only two complements while others showed no major difference in their adhesion. This study provides evidence of sequence variations across the different domains of SdrD from S. aureus isolated from healthy nasal carriers. Proper understanding of these variations is necessary in the study of S. aureus pathogenesis. The online version of this article (10.1186/s12866-018-1179-7) contains supplementary material, which is available to authorized users.
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影响因子：
4.2
作者：
Corrigan, Rebecca M.;Miajlovic, Helen;Foster, Timothy J.
通讯作者：
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影响因子：
11.8
作者：
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DOI：
10.1099/00221287-144-12-3387
发表时间：
1998-12-01
期刊：
MICROBIOLOGY-UK
影响因子：
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影响因子：
3.2
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影响因子：
11.4
作者：
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通讯作者：
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