Direct capture and smartphone quantification of airborne SARS-CoV-2 on a paper microfluidic chip.
Direct capture and smartphone quantification of airborne SARS-CoV-2 on a paper microfluidic chip.
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DOI：
10.1016/j.bios.2021.113912
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发表时间：
2022-03-15
影响因子：
12.6
通讯作者：
Yoon JY
中科院分区：
文献类型：
--
作者：
Kim S;Akarapipad P;Nguyen BT;Breshears LE;Sosnowski K;Baker J;Uhrlaub JL;Nikolich-Žugich J;Yoon JY
SARS是由SARS-CoV引起的一种新型呼吸道疾病，于2003年被发现，发病率和死亡率很高。最近由SARS-CoV-2引起的COVID-19大流行在全球范围内造成了更大程度的发病率和死亡率。SARS-CoV和SARS-CoV-2都通过呼气、咳嗽和打喷嚏以飞沫和可能更小的飞沫（气溶胶）的形式在空气中传播。从这种空气飞沫中直接检测将是保护公众在感染个体之前免受潜在暴露的理想方法。然而，这种液滴和气溶胶中的病毒数量太少，无法直接检测。需要单独的空气采样器和足够的收集时间（几个小时）来捕获足够数量的病毒。在这项工作中，我们已经证明了在纸微流控芯片上直接捕获空气中的液滴，而不需要任何其他设备。10%的人唾液样本被掺入已知浓度的SARS-CoV-2，并喷洒到空气中产生液滴和气溶胶。然后将抗体结合的亚微米颗粒悬浮液加入纸通道中，基于智能手机的荧光显微镜分离并计数纸芯片上的免疫凝集颗粒。总捕获到测定时间<30分钟，而其他方法需要数小时。这样，SARS-CoV-2就可以以手持和低成本的方式直接从空气中检测到，有助于减缓SARS-CoV-2的传播。我们大概可以将这项技术应用于其他呼吸道病毒。
SARS, a new type of respiratory disease caused by SARS-CoV, was identified in 2003 with significant levels of morbidity and mortality. The recent pandemic of COVID-19, caused by SARS-CoV-2, has generated even greater extents of morbidity and mortality across the entire world. Both SARS-CoV and SARS-CoV-2 spreads through the air in the form of droplets and potentially smaller droplets (aerosols) via exhaling, coughing, and sneezing. Direct detection from such airborne droplets would be ideal for protecting general public from potential exposure before they infect individuals. However, the number of viruses in such droplets and aerosols is too low to be detected directly. A separate air sampler and enough collection time (several hours) are necessary to capture a sufficient number of viruses. In this work, we have demonstrated the direct capture of the airborne droplets on the paper microfluidic chip without the need for any other equipment. 10% human saliva samples were spiked with the known concentration of SARS-CoV-2 and sprayed to generate liquid droplets and aerosols into the air. Antibody-conjugated submicron particle suspension is then added to the paper channel, and a smartphone-based fluorescence microscope isolated and counted the immunoagglutinated particles on the paper chip. The total capture-to-assay time was <30 min, compared to several hours with the other methods. In this manner, SARS-CoV-2 could be detected directly from the air in a handheld and low-cost manner, contributing to slowing the spread of SARS-CoV-2. We can presumably adapt this technology to a wide range of other respiratory viruses.
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影响因子：
14.8
作者：
Chung, Soo;Breshears, Lane E.;Yoon, Jeong-Yeol
通讯作者：
Yoon, Jeong-Yeol
DOI：
10.1073/pnas.2009637117
发表时间：
2020-06-30
影响因子：
11.1
作者：
Zhang, Renyi;Li, Yixin;Molina, Mario J.
通讯作者：
Molina, Mario J.
影响因子：
64.5
作者：
Fozouni P;Son S;Díaz de León Derby M;Knott GJ;Gray CN;D'Ambrosio MV;Zhao C;Switz NA;Kumar GR;Stephens SI;Boehm D;Tsou CL;Shu J;Bhuiya A;Armstrong M;Harris AR;Chen PY;Osterloh JM;Meyer-Franke A;Joehnk B;Walcott K;Sil A;Langelier C;Pollard KS;Crawford ED;Puschnik AS;Phelps M;Kistler A;DeRisi JL;Doudna JA;Fletcher DA;Ott M
通讯作者：
Ott M
影响因子：
--
作者：
Kwon, Hyuck-Jin;Fronczek, Christopher F.;Yoon, Jeong-Yeol
通讯作者：
Yoon, Jeong-Yeol
DOI：
10.1016/j.ijid.2020.12.035
发表时间：
2021-03
期刊：
International journal of infectious diseases : IJID : official publication of the International Society for Infectious Diseases
影响因子：
--
作者：
Hwang SE;Chang JH;Oh B;Heo J
通讯作者：
Heo J