Mobilizable genomic islands: going mobile with oriT mimicry.
Mobilizable genomic islands: going mobile with oriT mimicry.
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DOI：
10.1111/j.1365-2958.2010.07365.x
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发表时间：
2010-11
影响因子：
3.6
通讯作者：
Waldor MK
中科院分区：
文献类型：
--
作者：
Waldor MK
许多细菌染色体含有基因组岛，大的DNA片段在水平传播后并入染色体。然而，介导基因组岛横向转移的机制在很大程度上是未知的。在这一期的《分子微生物学》中，Daccord等人描述了一种新型的可传递的基因组岛，它可以通过共存的整合接合元件（ICE）来移动。这些“可移动的基因组岛”（MGI）需要许多ICE编码的因子用于其传输，包括诱导MGI切除的转录激活剂、在MGI oriT处启动转移的松弛酶和将MGI DNA运输至受体细胞的缀合机制。然而，MGIs编码它们自己的整合酶，这使得它们能够与新宿主的染色体重组，以及各种其他不具有与移动性相关的功能的基因。MGI oriT类似于ICE oriT，也可以作为ICE介导的大区域染色体DNA接合转移的起始位点。总的来说，这些发现提出了这样一种可能性，即染色体DNA从基因组岛内或染色体上其他地方的cyptic oriTs中的动员可能比以前认识到的更常见。
Many bacterial chromosomes contain genomic islands, large DNA segments that became incorporated into the chromosome following their horizontal transmission. However, the mechanisms that mediate the lateral transfer of genomic islands are for the most part unknown. In this issue of Molecular Microbiology, Daccord et al describe a new type of transmissible genomic island that can be mobilized by co-resident integrating conjugative elements (ICEs). These ‘mobilizable genomic islands’ (MGIs) require many ICE-encoded factors for their transmission, including transcription activators to induce MGI excision, the relaxase to initiate transfer at the MGI oriT and the conjugation machinery to transport MGI DNA to recipient cells. However, MGIs encode their own integrases, which enable their recombination with the chromosome of a new host, as well as a variety of other genes that do not have functions related to mobility. The MGI oriT, which resembles the ICE oriT, can also serve as a site for initiation of ICE-mediated conjugative transfer of large regions of chromosomal DNA. Overall, these findings raise the possibility that mobilization of chromosomal DNA from cyptic oriTs within genomic islands or elsewhere on the chromosome could be more common place than has been previously appreciated.
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影响因子：
3.6
作者：
Daccord, Aurelie;Ceccarelli, Daniela;Burrus, Vincent
通讯作者：
Burrus, Vincent
影响因子：
64.8
作者：
Beaber, JW;Hochhut, B;Waldor, MK
通讯作者：
Waldor, MK
影响因子：
3.2
作者：
Beaber, JW;Waldor, MK
通讯作者：
Waldor, MK
影响因子：
3.2
作者：
Meyer, Richard
通讯作者：
Meyer, Richard
毒素，毒素调节的菌毛和毒素调节剂对于人类的弧菌发病机理至关重要。
影响因子：
15.3
作者：
HERRINGTON, DA;HALL, RH;LOSONSKY, G;MEKALANOS, JJ;TAYLOR, RK;LEVINE, MM
通讯作者：
LEVINE, MM