Gene expression profiling of human decidual macrophages: evidence for immunosuppressive phenotype.
Gene expression profiling of human decidual macrophages: evidence for immunosuppressive phenotype.
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DOI：
10.1371/journal.pone.0002078
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发表时间：
2008-04-30
期刊：
影响因子：
3.7
通讯作者：
Ernerudh J
中科院分区：
文献类型：
--
作者：
Gustafsson C;Mjösberg J;Matussek A;Geffers R;Matthiesen L;Berg G;Sharma S;Buer J;Ernerudh J
虽然子宫巨噬细胞被认为在母胎界面上发挥着重要的调节作用，但它们的全球基因表达谱尚不清楚。利用包含约14,000个基因的微阵列，研究了人早孕蜕膜CD14+单核/巨噬细胞的基因表达谱，并与血液中相应细胞的表达谱进行了比较。一些关键的发现通过实时定量聚合酶链式反应或分泌蛋白得到了证实。早孕蜕膜CD14+细胞具有独特的基因表达模式。大量的调节基因在功能上与免疫调节和组织重塑有关，证实了分化的巨噬细胞的极化模式，主要是交替激活的M2表型。其中包括已知的M2标志物，如CCL-18、CD209、胰岛素样生长因子(IGF)-1、甘露糖受体C型(MRC)-1和纤维连接蛋白-1。此外，选择性地上调髓系细胞(TREM)-2、α-2-巨球蛋白(A2M)和前列腺素D2合成酶(PGDS)上表达的触发受体为了解妊娠蜕膜巨噬细胞的调节功能提供了新的见解，这可能与妊娠并发症有关。蜕膜巨噬细胞的分子特征显示了一个独特的转录图谱，其中充满了对胎儿免疫保护的重要成分，并为进一步研究这些细胞提供了几条线索。
Although uterine macrophages are thought to play an important regulatory role at the maternal-fetal interface, their global gene expression profile is not known. Using micro-array comprising approximately 14,000 genes, the gene expression pattern of human first trimester decidual CD14+ monocytes/macrophages was characterized and compared with the expression profile of the corresponding cells in blood. Some of the key findings were confirmed by real time PCR or by secreted protein. A unique gene expression pattern intrinsic of first trimester decidual CD14+ cells was demonstrated. A large number of regulated genes were functionally related to immunomodulation and tissue remodelling, corroborating polarization patterns of differentiated macrophages mainly of the alternatively activated M2 phenotype. These include known M2 markers such as CCL-18, CD209, insulin-like growth factor (IGF)-1, mannose receptor c type (MRC)-1 and fibronectin-1. Further, the selective up-regulation of triggering receptor expressed on myeloid cells (TREM)-2, alpha-2-macroglobulin (A2M) and prostaglandin D2 synthase (PGDS) provides new insights into the regulatory function of decidual macrophages in pregnancy that may have implications in pregnancy complications. The molecular characterization of decidual macrophages presents a unique transcriptional profile replete with important components for fetal immunoprotection and provides several clues for further studies of these cells.
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