The anticancer effects of ferulic acid is associated with induction of cell cycle arrest and autophagy in cervical cancer cells.
The anticancer effects of ferulic acid is associated with induction of cell cycle arrest and autophagy in cervical cancer cells.
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DOI：
10.1186/s12935-018-0595-y
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发表时间：
2018
影响因子：
5.8
通讯作者：
Wang Y
中科院分区：
文献类型：
--
作者：
Gao J;Yu H;Guo W;Kong Y;Gu L;Li Q;Yang S;Zhang Y;Wang Y
阿魏酸（4-羟基-3-甲氧基肉桂酸，FA）是从富含多酚化合物衍生的羟基肉桂酸。本研究旨在探讨阿魏酸（4-羟基-3-甲氧基肉桂酸; FA）对宫颈癌细胞系Hela和Caski细胞增殖、侵袭、凋亡和自噬的影响。MTT法检测FA对Hela和Caski细胞增殖的影响。Transwell法检测FA对Hela和Caski细胞的侵袭能力。RT-PCR检测MMP-9 mRNA表达情况。此外，使用流式细胞术检测细胞周期和细胞凋亡。Western blot分析细胞周期和自噬的7个蛋白的表达水平。FA（2.0mM）作用48 h后，对Hela和Caski细胞的抑制率分别为88.3%和85.4%。FA通过降低MMP-9 mRNA的表达而抑制细胞侵袭。FA使Hela和Caski细胞周期阻滞于G 0/G1期，并呈剂量依赖性（P < 0.05）。同时，FA诱导细胞周期相关蛋白p53、p21表达，降低Cyclin D1、Cyclin E水平。此外，FA还能剂量依赖性地降低自噬相关蛋白LC 3-II、Beclin 1和Atg 12-Atg 5的表达。FA可显著抑制Hela和Caski细胞的增殖和侵袭。它可能通过抑制人宫颈癌细胞自噬和诱导细胞周期阻滞而发挥抗肿瘤作用。
Ferulic acid (4-hydroxy-3-methoxycinnamic acid, FA) is a hydroxycinnamic acid derived from a rich polyphenolic compound. This study aimed to investigate the effect of ferulic acid (4-hydroxy-3-methoxycinnamic acid; FA) on cell proliferation, invasion, apoptosis, and autophagy in Hela and Caski cervical carcinoma cell lines. The cell proliferation of FA in Hela and Caski cells were detected by MTT assay. The cell invasion of FA in Hela and Caski cells were detected by Transwell assay. Subsequently, MMP-9 mRNA expression for cell invasion was detected by RT-PCR. Additionally, cell cycle and apoptosis were assayed using flow cytometry. Expression levels of 7 proteins for both cell cycle and autophagy were measured by Western blot analysis. After treated with FA (2.0 mM) for 48 h, the inhibition rates of FA in Hela and Caski cells were 88.3 and 85.4%, respectively. In addition, FA inhibited cell invasion through reducing MMP-9 mRNA expression. FA induced arrest in G0/G1 phase of the cell cycle in Hela and Caski cells with dose dependent (P < 0.05). Meanwhile, FA induced the cell cycle-related proteins expression such as p53 and p21, and reduced Cyclin D1 and Cyclin E levels. Moreover, FA decreased the autophagy-related proteins such as LC3-II, Beclin1 and Atg12-Atg5 in a dose-dependent manner. FA can significantly inhibit cell proliferation and invasion in Hela and Caski cells. It might be acted as an anti-cancer drug through inhibiting the autophagy and inducing cell cycle arrest in human cervical carcinoma cells.
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影响因子：
19
作者：
Funderburk SF;Wang QJ;Yue Z
通讯作者：
Yue Z
影响因子：
7.4
作者：
Magge RS;DeAngelis LM
通讯作者：
DeAngelis LM
影响因子：
6.4
作者：
de Martel C;Plummer M;Vignat J;Franceschi S
通讯作者：
Franceschi S
影响因子：
--
作者：
Eroglu, Canan;Secme, Mucahit;Dodurga, Yavuz
通讯作者：
Dodurga, Yavuz
影响因子：
3.5
作者：
Fang, Lei;Chen, Mohao;Chen, Li
通讯作者：
Chen, Li