Genome-Wide Analysis of Odorant and Gustatory Receptors in Six Papilio Butterflies (Lepidoptera: Papilionidae).
Genome-Wide Analysis of Odorant and Gustatory Receptors in Six Papilio Butterflies (Lepidoptera: Papilionidae).
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鳞翅目蝴蝶是食草昆虫的代表。寄主植物的多样性可能会影响蝴蝶化学感受器的进化。然而，很少有人知道是否寄主植物的范围是相关的化学感受器的大小差异的关键分子靶标蝴蝶植物化学相互作用。在这里，我们的特点是381气味（OR）和328味觉（GR）受体在一个凤蝶通才和其他五个凤蝶专家关于他们的全面注释，基因结构，遗传学和表达谱。在6种凤蝶中，orthopolysors或GRs共有保守的基因结构，但在通才和专才之间观察到内含子大小频率的差异。此外，表达谱确定候选OR和GR基因在触角，前跗骨或生殖组织的xuthus。本研究首次解决了寄主植物的呼吸似乎不会导致凤蝶中OR和GR的明显扩增的问题，并确定了与P. xuthus嗅觉，产卵或繁殖相关的候选分子靶标。昆虫和寄主植物的化学相互作用正在塑造化学感受器基因家族的进化。然而，宿主范围和化学感受器基因库大小之间的相关性仍然是难以捉摸的凤蝶科。在这里，我们解决的问题，是否寄主植物diramus相关的气味（ORs）或味觉（GRs）受体在6凤蝶的扩张。结合基因组学、转录组学和生物信息学方法，在一个通才灰绿拟青霉和五个专化拟青霉（P. xuthus、P. polytes、P. memnon、P. machaon和P. dardanus）的基因组中注释了381个OR和328个GR。凤蝶属中的Orthopolysors或GRs具有高度保守的基因结构。五个凤蝶专家表现出类似的频率内含子长度的OR或GR，但这是不同于那些在通才。系统发育分析显示，60个orthopathic OR组，其中45个共享一对一的关系。每只蝴蝶中的这种单一基因也出现在26个GR组中。有趣的是，苦味GR的内含子比其他GR少，并聚集成一个大的分支。针对柑橘P. xuthus的两个化学感受器基因家族，大多数PxutORs（52/58）在触角中表达，31个基因在生殖组织中表达。在28个前跗线表达的PxutGRs中，有11个是雌性偏向的基因，是感知产卵刺激物的强有力的候选基因。这些结果表明，寄主范围可能不会塑造大规模扩张的ORs和GRs在凤蝶和识别重要的分子靶标参与嗅觉，产卵或繁殖在P. xuthus。
The butterflies of the Lepidoptera are representatives of herbivorous insects. Host plant diversities may shape the evolution of chemosensory receptors in butterflies. However, little is known about whether the host plant range is correlated with the size differences of chemoreceptors as key molecular targets for butterfly–plant chemical interactions. Here, we characterized 381 odorant (ORs) and 328 gustatory (GRs) receptors in one Papilio generalist and five other Papilio specialists regarding their comprehensive annotation, gene structure, phylogenetics and expression profiles. Among six Papilio species, orthologous ORs or GRs shared conserved gene structure, but the differences in intron size frequencies were observed between the generalist and the specialists. Furthermore, expression profiles identified candidate OR and GR genes in antennae, foretarsi or reproductive tissues of P. xuthus. This study addresses, for the first time, the issue that the breath of host plants does not appear to result in the obvious expansions of ORs and GRs in Papilio butterflies and identifies candidate molecular targets associated with olfaction, oviposition or reproduction in P. xuthus. The chemical interactions of insects and host plants are shaping the evolution of chemosensory receptor gene families. However, the correlation between host range and chemoreceptor gene repertoire sizes is still elusive in Papilionidae. Here, we addressed the issue of whether host plant diversities are correlated with the expansions of odorant (ORs) or gustatory (GRs) receptors in six Papilio butterflies. By combining genomics, transcriptomics and bioinformatics approaches, 381 ORs and 328 GRs were annotated in the genomes of a generalist P. glaucus and five specialists, P. xuthus, P. polytes, P. memnon, P. machaon and P. dardanus. Orthologous ORs or GRs in Papilio had highly conserved gene structure. Five Papilio specialists exhibited a similar frequency of intron lengths for ORs or GRs, but which was different from those in the generalist. Phylogenetic analysis revealed 60 orthologous OR groups, 45 of which shared one-to-one relationships. Such a single gene in each butterfly also occurred in 26 GR groups. Intriguingly, bitter GRs had fewer introns than other GRs and clustered into a large clade. Focusing on the two chemoreceptor gene families in P. xuthus, most PxutORs (52/58) were expressed in antennae and 31 genes in reproductive tissues. Eleven out of 28 foretarsus-expressed PxutGRs were female-biased genes, as strong candidates for sensing oviposition stimulants. These results indicate that the host range may not shape the large-scale expansions of ORs and GRs in Papilio butterflies and identify important molecular targets involved in olfaction, oviposition or reproduction in P. xuthus.
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影响因子：
8.8
作者：
Cong Q;Borek D;Otwinowski Z;Grishin NV
通讯作者：
Grishin NV
影响因子：
64.8
作者：
Dasmahapatra, Kanchon K.;Walters, James R.;Briscoe, Adriana D.;Davey, John W.;Whibley, Annabel;Nadeau, Nicola J.;Zimin, Aleksey V.;Hughes, Daniel S. T.;Ferguson, Laura C.;Martin, Simon H.;Salazar, Camilo;Lewis, James J.;Adler, Sebastian;Ahn, Seung-Joon;Baker, Dean A.;Baxter, Simon W.;Chamberlain, Nicola L.;Chauhan, Ritika;Counterman, Brian A.;Dalmay, Tamas;Gilbert, Lawrence E.;Gordon, Karl;Heckel, David G.;Hines, Heather M.;Hoff, Katharina J.;Holland, Peter W. H.;Jacquin-Joly, Emmanuelle;Jiggins, Francis M.;Jones, Robert T.;Kapan, Durrell D.;Kersey, Paul;Lamas, Gerardo;Lawson, Daniel;Mapleson, Daniel;Maroja, Luana S.;Martin, Arnaud;Moxon, Simon;Palmer, William J.;Papa, Riccardo;Papanicolaou, Alexie;Pauchet, Yannick;Ray, David A.;Rosser, Neil;Salzberg, Steven L.;Supple, Megan A.;Surridge, Alison;Tenger-Trolander, Ayse;Vogel, Heiko;Wilkinson, Paul A.;Wilson, Derek;Yorke, James A.;Yuan, Furong;Balmuth, Alexi L.;Eland, Cathlene;Gharbi, Karim;Thomson, Marian;Gibbs, Richard A.;Han, Yi;Jayaseelan, Joy C.;Kovar, Christie;Mathew, Tittu;Muzny, Donna M.;Ongeri, Fiona;Pu, Ling-Ling;Qu, Jiaxin;Thornton, Rebecca L.;Worley, Kim C.;Wu, Yuan-Qing;Linares, Mauricio;Blaxter, Mark L.;Ffrench-Constant, Richard H.;Joron, Mathieu;Kronforst, Marcus R.;Mullen, Sean P.;Reed, Robert D.;Scherer, Steven E.;Richards, Stephen;Mallet, James;McMillan, W. Owen;Jiggins, Chris D.
通讯作者：
Jiggins, Chris D.
影响因子：
7.7
作者：
Bastin-Heline, Lucie;de Fouchier, Arthur;Montagne, Nicolas
通讯作者：
Montagne, Nicolas
DOI：
10.1073/pnas.0608424104
发表时间：
2007-03-20
影响因子：
11.1
作者：
McBride, Carolyn S.
通讯作者：
McBride, Carolyn S.
影响因子：
10.7
作者：
Katoh K;Standley DM
通讯作者：
Standley DM