Age and Sex Modulate SARS-CoV-2 Viral Load Kinetics: A Longitudinal Analysis of 1735 Subjects.
Age and Sex Modulate SARS-CoV-2 Viral Load Kinetics: A Longitudinal Analysis of 1735 Subjects.
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DOI：
10.3390/jpm11090882
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发表时间：
2021-09-02
影响因子：
--
通讯作者：
Giardina E
中科院分区：
文献类型：
--
作者：
Caputo V;Termine A;Fabrizio C;Calvino G;Luzzi L;Fusco C;Ingrascì A;Peconi C;D'Alessio R;Mihali S;Trastulli G;Megalizzi D;Cascella R;Rossini A;Salvia A;Strafella C;Giardina E
由 SARS-CoV-2 引起的 COVID-19 大流行代表了公共卫生紧急情况，由于高传染性变异体的检测和针对 COVID-19 的策略尚未明确制定，这变得更具挑战性。我们对原始毒株和变异毒株感染个体的时间病毒载量动力学进行了表征。对 2020 年 11 月至 2021 年 6 月期间 33,000 名个人（入住 IRCCS 圣卢西亚基金会免下车医院、医疗保健专业人员和接受常规筛查的住院患者）的鼻口咽拭子进行了分析。其中，选择了 1735 名受试者，并根据病毒株进行分组。通过基于 CE-IVD RT-PCR 的试剂盒进行诊断分析。使用数字 PCR 对 36 名受试者的亚基因组 RNA 成分进行了评估。通过广泛的统计分析评估和比较感染持续时间、病毒载量衰减速度、年龄和性别的影响。总体而言，各组之间的感染持续时间和病毒载量存在差异（p < 0.05）。在受高病毒载量影响且表现出快速衰减速度的原始病毒携带者和变异携带者中男性较多，而具有慢衰减速度的原始病毒携带者导致年龄较大（p < 0.05）。在阳性样本中检测到亚基因组 RNA，包括病毒载量较低的样本。这项研究提供了病毒载量动力学的图片，识别具有相似模式的个体并显示年龄和性别的不同影响，从而为感染受试者的个性化管理提供潜在有用的信息。
The COVID-19 pandemic caused by SARS-CoV-2 represents a public health emergency, which became even more challenging since the detection of highly transmissible variants and strategies against COVID-19 were indistinctly established. We characterized the temporal viral load kinetics in individuals infected by original and variant strains. Naso-oropharyngeal swabs from 33,000 individuals (admitted to the IRCCS Santa Lucia Foundation Drive-in, healthcare professionals and hospitalized patients who underwent routinary screening) from November 2020 to June 2021 were analyzed. Of them, 1735 subjects were selected and grouped according to the viral strain. Diagnostic analyses were performed by CE-IVD RT-PCR-based kits. The subgenomic-RNA component was assessed in 36 subjects using digital PCR. Infection duration, viral load decay speed, effects of age and sex were assessed and compared by extensive statistical analyses. Overall, infection duration and viral load differed between the groups (p < 0.05). Male sex was more present among both original and variant carriers affected with high viral load and showing fast decay speed, whereas original strain carriers with slow decay speed resulted in older (p < 0.05). Subgenomic-RNA was detected in the positive samples, including those with low viral load. This study provides a picture of the viral load kinetics, identifying individuals with similar patterns and showing differential effects of age and sex, thus providing potentially useful information for personalized management of infected subjects.
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DOI：
10.1016/s2468-2667(21)00055-4
发表时间：
2021-05
期刊：
The Lancet. Public health
影响因子：
--
作者：
Graham MS;Sudre CH;May A;Antonelli M;Murray B;Varsavsky T;Kläser K;Canas LS;Molteni E;Modat M;Drew DA;Nguyen LH;Polidori L;Selvachandran S;Hu C;Capdevila J;COVID-19 Genomics UK (COG-UK) Consortium;Hammers A;Chan AT;Wolf J;Spector TD;Steves CJ;Ourselin S
通讯作者：
Ourselin S
影响因子：
7.9
作者：
Klein SL;Passaretti C;Anker M;Olukoya P;Pekosz A
通讯作者：
Pekosz A
影响因子：
64.8
作者：
COVID-19 Host Genetics Initiative
通讯作者：
COVID-19 Host Genetics Initiative
影响因子：
7.8
作者：
Giefing-Kröll C;Berger P;Lepperdinger G;Grubeck-Loebenstein B
通讯作者：
Grubeck-Loebenstein B
影响因子：
4.8
作者：
Lai A;Bergna A;Menzo S;Zehender G;Caucci S;Ghisetti V;Rizzo F;Maggi F;Cerutti F;Giurato G;Weisz A;Turchi C;Bruzzone B;Ceccherini Silberstein F;Clementi N;Callegaro A;Sagradi F;Francisci D;Venanzi Rullo E;Vicenti I;Clementi M;Galli M;collaborative group SCIRE SARS-CoV-2 Italian Research Enterprise
通讯作者：
collaborative group SCIRE SARS-CoV-2 Italian Research Enterprise