Examining the Role of Microbiota in Emotional Behavior: Antibiotic Treatment Exacerbates Anxiety in High Anxiety-Prone Male Rats.
Examining the Role of Microbiota in Emotional Behavior: Antibiotic Treatment Exacerbates Anxiety in High Anxiety-Prone Male Rats.
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DOI：
10.1016/j.neuroscience.2021.01.030
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发表时间：
2021-04-01
期刊：
影响因子：
3.3
通讯作者：
Clinton SM
中科院分区：
文献类型：
--
作者：
Glover ME;Cohen JL;Singer JR;Sabbagh MN;Rainville JR;Hyland MT;Morrow CD;Weaver CT;Hodes GE;Kerman IA;Clinton SM
肠道微生物群对于健康的胃肠道功能至关重要，并且还通过免疫功能的变化广泛影响大脑功能和行为。胃肠道疾病与精神疾病高度共病，尽管对这些疾病的生物学机制知之甚少。本研究利用为不同的情绪行为表型而饲养的大鼠来研究情绪性、微生物组和免疫标志物之间的关系。先前的工作表明，低新奇反应（LR）大鼠表现出高水平的焦虑和抑郁相关的行为，以及无数的神经生物学差异相比，高新奇反应（HR）。在这里，我们假设不同的HR/LR表型伴随着粪便微生物组组成的变化。我们使用下一代测序来评估HR/LR微生物组，然后用抗生素鸡尾酒治疗成年HR/LR男性，以测试它是否改变了行为。鉴于微生物组与免疫系统之间的已知联系，我们还分析了循环细胞因子和代谢因子，以确定外周免疫标志物、肠道微生物组成分和行为指标之间的关系。没有基线HR/LR微生物组差异，抗生素治疗破坏了HR和LR大鼠的微生物组。抗生素治疗加剧了HR/LR行为的各个方面，增加了LR已经很高的焦虑样行为水平，同时减少了两种菌株的被动压力应对。我们的研究结果强调了个体表型对抗生素反应的重要性，有助于理解肠道微生物，免疫功能和个体情感表型之间复杂的相互作用。
Intestinal microbiota are essential for healthy gastrointestinal function and also broadly influence brain function and behavior, in part, through changes in immune function. Gastrointestinal disorders are highly comorbid with psychiatric disorders, although biological mechanisms linking these disorders are poorly understood. The present study utilized rats bred for distinct emotional behavior phenotypes to examine relationships between emotionality, the microbiome, and immune markers. Prior work showed that Low Novelty Responder (LR) rats exhibit high levels of anxiety- and depression-related behaviors as well as myriad neurobiological differences compared to High Novelty Responders (HRs). Here, we hypothesized that the divergent HR/LR phenotypes are accompanied by changes in fecal microbiome composition. We used next-generation sequencing to assess the HR/LR microbiomes and then treated adult HR/LR males with an antibiotic cocktail to test whether it altered behavior. Given known connections between the microbiome and immune system, we also analyzed circulating cytokines and metabolic factors to determine relationships between peripheral immune markers, gut microbiome components, and behavioral measures. There were no baseline HR/LR microbiome differences, and antibiotic treatment disrupted the microbiome in both HR and LR rats. Antibiotic treatment exacerbated aspects of HR/LR behavior, increasing LRs’ already high levels of anxiety-like behavior while reducing passive stress coping in both strains. Our results highlight the importance of an individual’s phenotype to their response to antibiotics, contributing to the understanding of the complex interplay between gut microbes, immune function, and an individual’s emotional phenotype.
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DOI：
10.1111/ejn.13624
发表时间：
2017-10
期刊：
The European journal of neuroscience
影响因子：
--
作者：
Cohen JL;Jackson NL;Ballestas ME;Webb WM;Lubin FD;Clinton SM
通讯作者：
Clinton SM
影响因子：
10.6
作者：
Birt IA;Hagenauer MH;Clinton SM;Aydin C;Blandino P Jr;Stead JDH;Hilde KL;Meng F;Thompson RC;Khalil H;Stefanov A;Maras P;Zhou Z;Hebda-Bauer EK;Goldman D;Watson SJ Jr;Akil H
通讯作者：
Akil H
影响因子：
--
作者：
Banawas SS
通讯作者：
Banawas SS
影响因子：
4.6
作者：
Corem, Noa;Anzi, Shira;Ben-Zvi, Ayal
通讯作者：
Ben-Zvi, Ayal
影响因子：
3.7
作者：
Clinton, Sarah;Miller, Sue;Akil, Huda
通讯作者：
Akil, Huda