Confocal fluorescence imaging enables noninvasive quantitative assessment of host cell populations in vivo following photodynamic therapy.
Confocal fluorescence imaging enables noninvasive quantitative assessment of host cell populations in vivo following photodynamic therapy.
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共聚焦荧光成像可以在光动力疗法后对体内的宿主细胞群进行无创的定量评估。
DOI：
10.7150/thno.4385
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发表时间：
2012
期刊：
影响因子：
12.4
通讯作者：
Foster TH
中科院分区：
文献类型：
--
作者：
Mitra S;Mironov O;Foster TH
我们报道了利用光学成像技术非侵入性地检测BALB/c小鼠EMT6肿瘤的光敏剂分布以及对2-[1-己氧乙基]-2去乙烯焦脱镁叶绿酸(HPPH)介导的光动力疗法(PDT)的生理和宿主反应。静脉注射1μ摩尔·kg~(-1)HPPH 24小时后，广野荧光成像显示肿瘤的选择性，肿瘤与邻近正常组织之间的差异约为2-3倍。共聚焦显微镜显示瘤内HPPH分布相对均匀。用荧光团标记的抗体标记宿主细胞，可以在体内显示Gr1+/CD11b+白细胞和主要组织相容性复合体II类(MHC-II)+细胞。放射治疗后不同时间点的治疗部位成像显示，Gr1+细胞对治疗的反应显著且迅速增加。Gr1+细胞在照射后24 h达到最大聚集量，48 h后下降。使用静脉注射FITC标记的葡聚糖作为荧光灌注标记物，我们对照射后不同时间的组织灌注进行成像，发现48h时Gr1+细胞密度的降低与血管系统的功能损伤密切相关，据报道，这种损害是通过灌注减少来实现的。双色共聚焦成像实验表明，约90%的抗Gr1细胞群与抗CD11b标记的细胞共定位，从而表明大多数Gr1标记的细胞是中性粒细胞。在PDT后24小时，与未经处理的对照组相比，MHC-II+细胞大约增加了2倍。共定位分析显示，表达MHC-II的Gr1+细胞比例增加，提示HPPH-PDT正在刺激中性粒细胞表达抗原提呈表型。
We report the use of optical imaging strategies to noninvasively examine photosensitizer distribution and physiological and host responses to 2-[1-hexyloxyethyl]-2 devinyl pyropheophorbide-a (HPPH)-mediated photodynamic therapy (PDT) of EMT6 tumors established in the ears of BALB/c mice. 24 h following intravenous (IV) administration of 1 μmol kg-1 HPPH, wide-field fluorescence imaging reveals tumor selectivity with an approximately 2-3-fold differential between tumor and adjacent normal tissue. Confocal microscopy demonstrates a relatively homogeneous intratumor HPPH distribution. Labeling of host cells using fluorophore-conjugated antibodies allowed the visualization of Gr1+/CD11b+ leukocytes and major histocompatibility complex class II (MHC-II)+ cells in vivo. Imaging of the treated site at different time-points following irradiation shows significant and rapid increases in Gr1+ cells in response to therapy. The maximum accumulation of Gr1+ cells is found at 24 h post-irradiation, followed by a decrease at the 48 h time-point. Using IV-injected FITC-conjugated dextran as a fluorescent perfusion marker, we imaged tissue perfusion at different times post-irradiation and found that the reduced Gr1+ cell density at 48 h correlated strongly with functional damage to the vasculature as reported via decreased perfusion status. Dual color confocal imaging experiments demonstrates that about 90% of the anti-Gr1 cell population co-localized with anti-CD11b labeling, thus indicating that majority of the Gr1-labeled cells were neutrophils. At 24 h post-PDT, an approximately 2-fold increase in MHC-II+ cells relative to untreated control is also observed. Co-localization analysis reveals an increase in the fraction of Gr1+ cells expressing MHC-II, suggesting that HPPH-PDT is stimulating neutrophils to express an antigen-presenting phenotype.
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影响因子：
2.4
作者：
Nava, Hector R.;Allamaneni, Shyam S.;Dougherty, Thomas J.;Cooper, Michele T.;Tan, Wei;Wilding, Gregory;Henderson, Barbara W.
通讯作者：
Henderson, Barbara W.
DOI：
10.3322/caac.20114
发表时间：
2011-07
期刊：
CA: a cancer journal for clinicians
影响因子：
--
作者：
Agostinis P;Berg K;Cengel KA;Foster TH;Girotti AW;Gollnick SO;Hahn SM;Hamblin MR;Juzeniene A;Kessel D;Korbelik M;Moan J;Mroz P;Nowis D;Piette J;Wilson BC;Golab J
通讯作者：
Golab J
影响因子：
2.4
作者：
Mitra, Soumya;Haidaris, Constantine G.;Snell, Sara B.;Giesselman, Benjamin R.;Hupcher, Steven M.;Foster, Thomas H.
通讯作者：
Foster, Thomas H.
影响因子：
2.4
作者：
Lobel, J;MacDonald, IJ;Dougherty, TJ
通讯作者：
Dougherty, TJ
影响因子：
11.5
作者：
Chen, B;Pogue, BW;Hasan, T
通讯作者：
Hasan, T