The development of murine plasmacytoid dendritic cell precursors is differentially regulated by FLT3-ligand and granulocyte/macrophage colony-stimulating factor.
The development of murine plasmacytoid dendritic cell precursors is differentially regulated by FLT3-ligand and granulocyte/macrophage colony-stimulating factor.
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DOI：
10.1084/jem.20020045
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发表时间：
2002-04-01
期刊：
影响因子：
--
通讯作者：
Liu YJ
中科院分区：
文献类型：
--
作者：
Gilliet M;Boonstra A;Paturel C;Antonenko S;Xu XL;Trinchieri G;O'Garra A;Liu YJ
最近在小鼠中确定了浆细胞样易易易易易易易易点细胞或1型干扰素（IFN）产生的细胞（IPC）。他们的一代仍然未知。通过对比度GM-CSF或TNF-α的小鼠骨髓培养物，促进CD11C+CD11B+B220-髓样DC的产生，完全阻止了IPC的发展。例如浆细胞样形态，在对单纯疱疹病毒的反应中产生大量IFN-α的能力，以及对Toll样受体9的配体反应的能力（TLR-9; CpG ODN 1668），但不适合TLR-4的配体（脂多糖[LPS]），与人类IPC产生的IL-12P70不同，小鼠IPC会响应CPG ODN 1668和疱疹病毒鼠CD11C+CD11B-B220+GR-1+IPC和CD11C+CD11B+B220-髓样DC的开发受FLT3-Ligand和粒细胞/巨噬细胞刺激因子和小鼠IPC显示出不同的IL-12P70的能力。
Plasmacytoid predendritic cells or type 1 interferon (IFN)-producing cells (IPCs) have recently been identified in mice. Although culture systems giving rise to different murine dendritic cell subsets have been established, the developmental regulation of murine plasmacytoid IPCs and the culture conditions leading to their generation remain unknown. Here we show that large numbers of over 40% pure CD11c+CD11b−B220+Gr-1+ IPCs can be generated from mouse bone marrow cultures with FLT3-ligand. By contrast GM-CSF or TNF-α, which promote the generation of CD11c+CD11b+B220− myeloid DCs, block completely the development of IPCs. IPCs generated display similar features to human IPCs, such as the plasmacytoid morphology, the ability to produce large amounts of IFN-α in responses to herpes simplex virus, and the capacity to respond to ligands for Toll-like receptor 9 (TLR-9; CpG ODN 1668), but not to ligands for TLR-4 (lipopolysaccharide [LPS]). Unlike human IPCs which produce little IL-12p70, mouse IPCs produce IL-12p70 in response to CpG ODN 1668 and herpes simplex virus. This study demonstrates that the development of murine CD11c+CD11b−B220+Gr-1+ IPCs and CD11c+CD11b+B220− myeloid DCs is differentially regulated by FLT3-ligand and granulocyte/macrophage colony-stimulating factor. Human IPCs and mouse IPCs display different ability to produce IL-12p70. Large numbers of mouse IPCs can now be obtained from total bone marrow culture.
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影响因子：
30.5
作者：
Cella, M;Facchetti, F;Colonna, M
通讯作者：
Colonna, M
影响因子：
30.5
作者：
Farrar, JD;Smith, JD;Murphy, KM
通讯作者：
Murphy, KM
影响因子：
4.4
作者：
Dzionek, A;Fuchs, A;Schmitz, J
通讯作者：
Schmitz, J
DOI：
10.1084/jem.185.6.1101
发表时间：
1997-03-17
期刊：
The Journal of experimental medicine
影响因子：
--
作者：
Grouard G;Rissoan MC;Filgueira L;Durand I;Banchereau J;Liu YJ
通讯作者：
Liu YJ
DOI：
10.1084/jem.184.6.2185
发表时间：
1996-12-01
期刊：
The Journal of experimental medicine
影响因子：
--
作者：
Saunders D;Lucas K;Ismaili J;Wu L;Maraskovsky E;Dunn A;Shortman K
通讯作者：
Shortman K