A Novel "Off-On" Fluorescent Probe Based on Carbon Nitride Nanoribbons for the Detection of Citrate Anion and Live Cell Imaging.

A Novel "Off-On" Fluorescent Probe Based on Carbon Nitride Nanoribbons for the Detection of Citrate Anion and Live Cell Imaging.
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DOI:
10.3390/s18041163
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发表时间:
2018-04-11
期刊:
Sensors (Basel, Switzerland)
影响因子:
--
通讯作者:
Wang L
Wang L
中科院分区:
其他
文献类型:
--
作者:
Hu Y;Yang D;Yang C;Feng N;Shao Z;Zhang L;Wang X;Weng L;Luo Z;Wang L

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研制了一种基于氮化碳(C3N4)纳米带的新型柠檬酸盐阴离子(C6H5O73−)荧光“关通”探针。C3N4纳米带的荧光可以被Cu2+猝灭,然后通过加入C6H5O73−恢复,这是因为C6H5O73−与Cu2+之间的络合作用阻碍了Cu2+和C3N4纳米带之间的电子转移。使用该荧光“开-开”探针的开通型荧光传感器可以快速、选择性地检测C6H5O73−,在水溶液中具有较宽的检测线性范围(1~400μM)和低检测下限(0.78μM)。重要的是,这种基于C3N4纳米带的“OFF-ON”探针具有良好的生物相容性,可以作为外源性C6H5O73−在HeLa细胞中的荧光显像剂。
A novel fluorescent “off-on” probe based on carbon nitride (C3N4) nanoribbons was developed for citrate anion (C6H5O73−) detection. The fluorescence of C3N4 nanoribbons can be quenched by Cu2+ and then recovered by the addition of C6H5O73−, because the chelation between C6H5O73− and Cu2+ blocks the electron transfer between Cu2+ and C3N4 nanoribbons. The turn-on fluorescent sensor using this fluorescent “off-on” probe can detect C6H5O73− rapidly and selectively, showing a wide detection linear range (1~400 μM) and a low detection limit (0.78 μM) in aqueous solutions. Importantly, this C3N4 nanoribbon-based “off-on” probe exhibits good biocompatibility and can be used as fluorescent visualizer for exogenous C6H5O73− in HeLa cells.
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