Optical-flow based non-invasive analysis of cardiomyocyte contractility.
Optical-flow based non-invasive analysis of cardiomyocyte contractility.
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DOI：
10.1038/s41598-017-10094-7
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发表时间：
2017-09-04
影响因子：
4.6
通讯作者：
Rajasingh J
中科院分区：
文献类型：
--
作者：
Czirok A;Isai DG;Kosa E;Rajasingh S;Kinsey W;Neufeld Z;Rajasingh J
心肌细胞搏动模式的表征对于用于外科注射的细胞的质量控制以及在疾病建模或毒性研究中建立表型是需要的。基于光流的视频显微镜记录分析提供了一个免操作和有效的表征收缩周期，心肌细胞表型的重要特征。我们表明，通过适当的计算分析的光流数据，可以识别不同的收缩中心，并区分主动细胞收缩被动弹性组织变形。我们提出的收敛措施与肌球蛋白IIa免疫定位，并能够解决收缩波和他们的同步成熟，未标记的诱导多能干细胞衍生的心肌细胞培养。
Characterization of cardiomyocyte beat patterns is needed for quality control of cells intended for surgical injection as well as to establish phenotypes in disease modeling or toxicity studies. Optical-flow based analysis of videomicroscopic recordings offer a manipulation-free and efficient characterization of contractile cycles, an important characteristics of cardiomyocyte phenotype. We demonstrate that by appropriate computational analysis of optical flow data one can identify distinct contractile centers and distinguish active cell contractility from passive elastic tissue deformations. Our proposed convergence measure correlates with myosin IIa immuno-localization and is capable to resolve contractile waves and their synchronization within maturing, unlabeled induced pluripotent stem cell-derived cardiomyocyte cultures.
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影响因子：
37.8
作者：
Go AS;Mozaffarian D;Roger VL;Benjamin EJ;Berry JD;Blaha MJ;Dai S;Ford ES;Fox CS;Franco S;Fullerton HJ;Gillespie C;Hailpern SM;Heit JA;Howard VJ;Huffman MD;Judd SE;Kissela BM;Kittner SJ;Lackland DT;Lichtman JH;Lisabeth LD;Mackey RH;Magid DJ;Marcus GM;Marelli A;Matchar DB;McGuire DK;Mohler ER 3rd;Moy CS;Mussolino ME;Neumar RW;Nichol G;Pandey DK;Paynter NP;Reeves MJ;Sorlie PD;Stein J;Towfighi A;Turan TN;Virani SS;Wong ND;Woo D;Turner MB;American Heart Association Statistics Committee and Stroke Statistics Subcommittee
通讯作者：
American Heart Association Statistics Committee and Stroke Statistics Subcommittee
影响因子：
23.9
作者：
Burridge, Paul W.;Keller, Gordon;Gold, Joseph D.;Wu, Joseph C.
通讯作者：
Wu, Joseph C.
影响因子：
2.7
作者：
Aleksandrova A;Czirók A;Szabó A;Filla MB;Hossain MJ;Whelan PF;Lansford R;Rongish BJ
通讯作者：
Rongish BJ
影响因子：
17.1
作者：
Matsa E;Burridge PW;Wu JC
通讯作者：
Wu JC
影响因子：
48
作者：
Burridge, Paul W.;Matsa, Elena;Shukla, Praveen;Lin, Ziliang C.;Churko, Jared M.;Ebert, Antje D.;Lan, Feng;Diecke, Sebastian;Huber, Bruno;Mordwinkin, Nicholas M.;Plews, Jordan R.;Abilez, Oscar J.;Cui, Bianxiao;Gold, Joseph D.;Wu, Joseph C.
通讯作者：
Wu, Joseph C.