Responses of phosphate transporter gene and alkaline phosphatase in Thalassiosira pseudonana to phosphine.
Responses of phosphate transporter gene and alkaline phosphatase in Thalassiosira pseudonana to phosphine.
复制标题
Thalassiosira pseudonana中磷酸转运蛋白基因和碱性磷酸酶对磷酸的反应。
DOI：
10.1371/journal.pone.0059770
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Liu Y
中科院分区：
文献类型：
--
作者：
Fu M;Song X;Yu Z;Liu Y
沉积物中不断释放的磷化氢能够影响浮游植物的生理生态策略和分子响应。为探讨磷化氢对海链藻磷吸收利用的影响，研究了不同浓度磷化氢对海链藻磷酸盐转运蛋白基因（TpPHO）转录水平和碱性磷酸酶（AKP）活性的影响。TpPHO表达在磷酸盐缺乏和磷酸盐-4 µM培养物中均被膦显著促进。然而，高浓度的磷化氢可以抑制TpPHO转录在下降的生长阶段。AKP活性也高于对照组。在0至0.056 µM范围内，它随磷化氢浓度的增加而增加，但被较高水平的磷化氢抑制。这些反应表明磷化氢可以影响T.是的这一结果与磷化氢对藻类生长的影响一致，而TpPHO表达和AKP对磷化氢的敏感性甚至高于藻类生长。本研究为进一步研究磷化氢对浮游植物的影响提供了基础。
Phosphine, which is released continuously from sediment, can affect the eco-physiological strategies and molecular responses of phytoplankton. To examine the effects of phosphine on phosphorus uptake and utilization in Thalassiosira pseudonana, we examined the transcriptional level of the phosphate transporter gene (TpPHO) and the activity of alkaline phosphatase (AKP) in relation to supplement of various concentrations of phosphine. TpPHO expression was markedly promoted by phosphine in both the phosphate-deficient and phosphate-4 µM culture. However, high phosphine concentrations can inhibit TpPHO transcription in the declining growth phase. AKP activity was also higher in the phosphine treatment groups than that of the control. It increased with increasing phosphine concentration in the range of 0 to 0.056 µM but was inhibited by higher levels of phosphine. These responses revealed that phosphine can affect phosphate uptake and utilization in T. pseudonana. This result was consistent with the effect of phosphine on algal growth, while TpPHO expression and AKP were even more sensitive to phosphine than algal growth. This work provides a basic understanding for further research about how phosphine affects phytoplankton.
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