Transcriptomic Analysis Reveals Panicle Heterosis in an Elite Hybrid Rice ZZY10 and Its Parental Lines.
Transcriptomic Analysis Reveals Panicle Heterosis in an Elite Hybrid Rice ZZY10 and Its Parental Lines.
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DOI：
10.3390/plants12061309
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发表时间：
2023-03-14
期刊：
影响因子：
--
通讯作者：
Tong H
中科院分区：
文献类型：
--
作者：
Zhong Z;Wu Y;Zhang P;Hu G;Fu D;Yu G;Tong H
杂种优势是指杂种的某些性状优于亲本的上级性状的现象。大多数研究分析了作物农艺性状的杂种优势，然而，穗部杂种优势可以提高产量，对作物育种具有重要意义。因此，需要对穗部杂种优势进行系统的研究，特别是在生殖生长阶段。RNA测序和转录组分析是进一步研究杂种优势的有效手段。利用Illumina Nova Seq平台，分析了优良杂交稻中浙优10号（ZZY 10）、保持系中浙B（ZZB）和恢复系Z7-10在2022年杭州抽穗期的转录组。通过测序获得了5.81亿个高质量短读段，并与日本晴参考基因组进行了比对。共发现9000个差异表达基因。在DGHP基因中，有60.71%的DGHP基因在杂交后代中表达上调，39.29%的DGHP基因表达下调。比较转录组分析表明，ZZY 10与中浙B之间和ZZY 10与Z7-10之间分别有5235和3765个DGHP。该结果与ZZY 10的转录组谱一致，并且与Z7-10相似。DGHP基因的表达模式主要表现为超显性、欠显性和加性。在DGHP参与的GO术语中，光合作用、DNA整合、细胞壁修饰、类囊体和光系统等途径是重要的。选择21个参与光合作用的DGHP和17个随机DGHP进行qRT-PCR验证。在本研究中，我们观察到在光合途径中PsbQ的上调和PSI和PSII亚基以及光合电子传递的下调。通过RNA-Seq获得了大量的转录组数据，提供了杂种优势杂交种抽穗期穗部转录组的全面概述。
Heterosis is the phenomenon in which some hybrid traits are superior to those of their parents. Most studies have analyzed the heterosis of agronomic traits of crops; however, heterosis of the panicles can improve yield and is important for crop breeding. Therefore, a systematic study of panicle heterosis is needed, especially during the reproductive stage. RNA sequencing (RNA Seq) and transcriptome analysis are suitable for further study of heterosis. Using the Illumina Nova Seq platform, the transcriptome of ZhongZheYou 10 (ZZY10), an elite rice hybrid, the maintainer line ZhongZhe B (ZZB), and the restorer line Z7-10 were analyzed at the heading date in Hangzhou, 2022. 581 million high-quality short reads were obtained by sequencing and were aligned against the Nipponbare reference genome. A total of 9000 differential expression genes were found between the hybrids and their parents (DGHP). Of the DGHP, 60.71% were up-regulated and 39.29% were down-regulated in the hybrid. Comparative transcriptome analysis revealed that 5235 and 3765 DGHP were between ZZY10 and ZhongZhe B and between ZZY10 and Z7-10, respectively. This result is consistent with the transcriptome profile of ZZY10 and was similar to Z7-10. The expression patterns of DGHP mainly exhibited over-dominance, under-dominance, and additivity. Among the DGHP-involved GO terms, pathways such as photosynthesis, DNA integration, cell wall modification, thylakoid, and photosystem were significant. 21 DGHP, which were involved in photosynthesis, and 17 random DGHP were selected for qRT-PCR validation. The up-regulated PsbQ and down-regulated subunits of PSI and PSII and photosynthetic electron transport in the photosynthesis pathway were observed in our study. Extensive transcriptome data were obtained by RNA-Seq, providing a comprehensive overview of panicle transcriptomes at the heading stage in a heterotic hybrid.
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