PAP90, a novel rice protein plays a critical role in regulation of D1 protein stability of PSII.
PAP90, a novel rice protein plays a critical role in regulation of D1 protein stability of PSII.
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DOI：
10.1016/j.jare.2020.11.008
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发表时间：
2021-05
影响因子：
10.7
通讯作者：
Balachandran SM
中科院分区：
文献类型：
--
作者：
Reddy MR;Mangrauthia SK;Reddy SV;Manimaran P;Yugandhar P;Babu PN;Vishnukiran T;Subrahmanyam D;Sundaram RM;Balachandran SM
光系统II（PSII）蛋白复合体在整个光合作用过程中起着重要的作用。各种已知和未知的蛋白质因子参与PSII复合物的动力学，需要在作物植物中表征以提高光合作用效率和生产力。本实验旨在研究水稻PSII动态的调控蛋白。水稻新的调节蛋白PAP 90（PSII辅助蛋白~90 kDa）的特征在于产生了一个功能丧失的突变体pap 90。在分子水平上对突变体进行了表征，并通过各种实验分析了突变体在控制和非生物胁迫下的形态、生理和生化过程。pap 90突变体表现出减少光合作用，由于D1蛋白的不稳定性，随后导致类囊体膜复合物，特别是PSII的积累不足，降低PSII的功能效率。在突变体中诱导OsFtsH家族基因和蛋白的表达，已知其在D1蛋白降解和周转中起关键作用。突变体中D1蛋白积累的减少增加了活性氧（ROS）的产生。pap 90突变体的抗氧化酶活性增加，诱导了胁迫相关基因和蛋白的表达，从而沿着ROS的积累，增强了其对水分胁迫的耐受能力。我们建议，PAP 90是一个关键的辅助蛋白，与D1蛋白相互作用，并保持其稳定性，从而促进随后的组装PSII和相关的膜复合物。
Photosystem II (PSII) protein complex plays an essential role in the entire photosynthesis process. Various known and unknown protein factors are involved in the dynamics of the PSII complex that need to be characterized in crop plants for enhancing photosynthesis efficiency and productivity. The experiments were conducted to decipher the regulatory proteins involved in PSII dynamics of rice crop. A novel rice regulatory protein PAP90 (PSII auxiliary protein ~90 kDa) was characterized by generating a loss-of-function mutant pap90. The mutation was characterized at molecular level followed by various experiments to analyze the morphological, physiological and biochemical processes of mutant under control and abiotic stresses. The pap90 mutant showed reduced photosynthesis due to D1 protein instability that subsequently causes inadequate accumulation of thylakoid membrane complexes, especially PSII and decreases PSII functional efficiency. Expression of OsFtsH family genes and proteins were induced in the mutant, which are known to play a key role in D1 protein degradation and turnover. The reduced D1 protein accumulation in the mutant increased the production of reactive oxygen species (ROS). The accumulation of ROS along with the increased activity of antioxidant enzymes and induced expression of stress-associated genes and proteins in pap90 mutant contributed to its water-limited stress tolerance ability. We propose that PAP90 is a key auxiliary protein that interacts with D1 protein and maintains its stability, thereby promoting subsequent assembly of the PSII and associated membrane complexes.
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影响因子：
14.9
作者：
Huerta-Cepas J;Capella-Gutiérrez S;Pryszcz LP;Marcet-Houben M;Gabaldón T
通讯作者：
Gabaldón T
影响因子：
11.4
作者：
Fan X;Wu J;Chen T;Tie W;Chen H;Zhou F;Lin Y
通讯作者：
Lin Y
影响因子：
3.5
作者：
Fitzgerald, HA;Chern, MS;Ronald, PC
通讯作者：
Ronald, PC
影响因子：
6.9
作者：
DHINDSA, RS;PLUMBDHINDSA, P;THORPE, TA
通讯作者：
THORPE, TA
影响因子：
4.2
作者：
Dastur, RH;Desai, BL
通讯作者：
Desai, BL